Human MCM2 ELISA Kit

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  • Alternative name

    Human minichromosome maintenance protein 2 homolog ELISA Kit;Human nuclear protein BM28 ELISA Kit;Human BM28 ELISA Kit;Human CCNL1 ELISA Kit;Human CDCL1 ELISA Kit;Human KIAA0030 ELISA Kit;Human D3S3194 ELISA Kit;Human DFNA70 ELISA Kit;Human MITOTIN ELISA Kit;Human cdc19 ELISA Kit;Human minichromosome maintenance complex component 2 ELISA Kit;Human DNA replication licensing factor MCM2 ELISA Kit;Human cell devision cycle-like 1 ELISA Kit;Human cyclin-like 1 ELISA Kit;Human minichromosome maintenance deficient 2 (mitotin) ELISA Kit;

  • Catalog
    E024580
  • species
    Human
  • GeneMCM2
  • Standard CurveHuman MCM2 ELISA Kit
  • Other Species Mouse Mcm2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 2 (MCM2). No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 2 (MCM2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman MCM2 ELISA Kit allows for the in vitro quantitative determination of MCM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 2 (MCM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 2 (MCM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 2 (MCM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 2 (MCM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA replication licensing factor MCM2 Protein information
  • Uniprot ID MCM2_HUMAN
  • Uniprot AC P49736; Q14577; Q15023; Q8N2V1; Q969W7; Q96AE1; Q9BRM7;
  • UniGene Hs.477481;
  • GeneID 4171
  • KEGG hsa:4171;
  • Human DNA replication licensing factor MCM2 Protein SEQUENCE
  • SEQUENCE 904 AA; 101896 MW; 52C6DC61F128B404 CRC64;

    MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE

    DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDAY EAEGLALDDE

    DVEELTASQR EAAERAMRQR DREAGRGLGR MRRGLLYDSD EEDEERPARK

    RRQVERATED GEEDEEMIES IENLEDLKGH SVREWVSMAG PRLEIHHRFK

    NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR EHVLAYFLPE

    APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL

    HLNQLIRTSG VVTSCTGVLP QLSMVKYNCN KCNFVLGPFC QSQNQEVKPG

    SCPECQSAGP FEVNMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA

    DLVDSCKPGD EIELTGIYHN NYDGSLNTAN GFPVFATVIL ANHVAKKDNK

    VAVGELTDED VKMITSLSKD QQIGEKIFAS IAPSIYGHED IKRGLALALF

    GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS SRAIFTTGQG

    ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI

    HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT

    EPIISRFDIL CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KEEEGLANGS

    AAEPAMPNTY GVEPLPQEVL KKYIIYAKER VHPKLNQMDQ DKVAKMYSDL

    RKESMATGSI PITVRHIESM IRMAEAHARI HLRDYVIEDD VNMAIRVMLE

    SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV AEQVTYQRNR

    FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMNK FSHDLKRKMI

    LQQF

  • UCSC uc003ejp.5; human.;


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