Human MIB2 ELISA Kit

Alternative name
Human mind bomb homolog 2 ELISA Kit;Human novel zinc finger protein ELISA Kit;Human novelzin ELISA Kit;Human putative NF-kappa-B-activating protein 002N ELISA Kit;Human RING-type E3 ubiquitin transferase MIB2 ELISA Kit;Human skeletrophin ELISA Kit;Human zinc finger ZZ type with ankyrin repeat domain protein 1 ELISA Kit;Human SKD ELISA Kit;Human ZZANK1 ELISA Kit;Human ZZZ5 ELISA Kit;Human mindbomb E3 ubiquitin protein ligase 2 ELISA Kit;Human E3 ubiquitin-protein ligase MIB2 ELISA Kit;Human RING-type E3 ubiquitin transferase MIB2 ELISA Kit;Human mindbomb homolog 2 ELISA Kit;Human zinc finger, ZZ type with ankyrin repeat domain 1 ELISA Kit;
Catalog
E024563
species
Human
GeneMIB2
SpecificityThis assay has high sensitivity and excellent specificity for detection of human MIB2. No significant cross-reactivity or interference between human MIB2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MIB2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman MIB2 ELISA Kit allows for the in vitro quantitative determination of MIB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human E3 ubiquitin-protein ligase MIB2 Protein information
Uniprot ID MIB2_HUMAN
Uniprot AC Q8N911; Q8NB36; Q8NCY1; Q8NG59; Q8NG60; Q8NG61; Q8NI59; Q8WYN1;
UniGene Hs.135805; Hs.593430;
GeneID 142678
KEGG hsa:142678;
Human E3 ubiquitin-protein ligase MIB2 Protein SEQUENCE
SEQUENCE 1013 AA; 109939 MW; 737BA12300091C70 CRC64;
MGWKPSEARG QSQSFQASGL QPRSLKAARR ATGRPDRSRA ARPTMDPSAH
RSRAAPPNMD PDPQAGVQVG MRVVRGVDWK WGQQDGGEGG VGTVVELGRH
GSPSTPDRTV VVQWDQGTRT NYRAGYQGAH DLLLYDNAQI GVRHPNIICD
CCKKHGLRGM RWKCRVCLDY DLCTQCYMHN KHELAHAFDR YETAHSRPVT
LSPRQGLPRI PLRGIFQGAK VVRGPDWEWG SQDGGEGKPG RVVDIRGWDV
ETGRSVASVT WADGTTNVYR VGHKGKVDLK CVGEAAGGFY YKDHLPRLGK
PAELQRRVSA DSQPFQHGDK VKCLLDTDVL REMQEGHGGW NPRMAEFIGQ
TGTVHRITDR GDVRVQFNHE TRWTFHPGAL TKHHSFWVGD VVRVIGDLDT
VKRLQAGHGE WTDDMAPALG RVGKVVKVFG DGNLRVAVAG QRWTFSPSCL
VAYRPEEDAN LDVAERAREN KSSLSVALDK LRAQKSDPEH PGRLVVEVAL
GNAARALDLL RRRPEQVDTK NQGRTALQVA AYLGQVELIR LLLQARAGVD
LPDDEGNTAL HYAALGNQPE ATRVLLSAGC RADAINSTQS TALHVAVQRG
FLEVVRALCE RGCDVNLPDA HSDTPLHSAI SAGTGASGIV EVLTEVPNID
VTATNSQGFT LLHHASLKGH ALAVRKILAR ARQLVDAKKE DGFTALHLAA
LNNHREVAQI LIREGRCDVN VRNRKLQSPL HLAVQQAHVG LVPLLVDAGC
SVNAEDEEGD TALHVALQRH QLLPLVADGA GGDPGPLQLL SRLQASGLPG
SAELTVGAAV ACFLALEGAD VSYTNHRGRS PLDLAAEGRV LKALQGCAQR
FRERQAGGGA APGPRQTLGT PNTVTNLHVG AAPGPEAAEC LVCSELALLV
LFSPCQHRTV CEECARRMKK CIRCQVVVSK KLRPDGSEVA SAAPAPGPPR
QLVEELQSRY RQMEERITCP ICIDSHIRLV FQCGHGACAP CGSALSACPI
CRQPIRDRIQ IFV
UCSC uc001agg.4; human. [Q96AX9-1];

Category

Human MIB2 ELISA Kit