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Human MID1 ELISA Kit

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  • Alternative name

    Human MIDIN ELISA Kit;Human putative transcription factor XPRF ELISA Kit;Human RING finger protein 59 ELISA Kit;Human RING finger protein Midline-1 ELISA Kit;Human RING-type E3 ubiquitin transferase Midline-1 ELISA Kit;Human tripartite motif-containing protein 18 ELISA Kit;Human FXY ELISA Kit;Human RNF59 ELISA Kit;Human TRIM18 ELISA Kit;Human XPRF ELISA Kit;Human BBBG1 ELISA Kit;Human GBBB1 ELISA Kit;Human OGS1 ELISA Kit;Human OS ELISA Kit;Human OSX ELISA Kit;Human ZNFXY ELISA Kit;Human midline 1 ELISA Kit;Human E3 ubiquitin-protein ligase Midline-1 ELISA Kit;Human Opitz/BBB syndrome ELISA Kit;Human midline 1 RING finger protein ELISA Kit;Human tripartite motif protein TRIM18 ELISA Kit;Human zinc finger on X and Y, mouse, homolog of ELISA Kit;
  • Catalog
    E024538
  • species
    Human
  • GeneMID1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human MID1. No significant cross-reactivity or interference between human MID1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MID1 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman MID1 ELISA Kit allows for the in vitro quantitative determination of MID1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MID1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MID1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MID1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human E3 ubiquitin-protein ligase Midline-1 Protein information
  • Uniprot ID TRI18_HUMAN
  • Uniprot AC O15344; B2RCG2; O75361; Q9BZX5;
  • UniGene Hs.27695; Hs.460482; Hs.689953; Hs.738954;
  • GeneID 4281
  • KEGG hsa:4281;
  • Human E3 ubiquitin-protein ligase Midline-1 Protein SEQUENCE

  • SEQUENCE 667 AA; 75251 MW; 673C5120018BA619 CRC64;

    METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNESVES

    ITAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET

    RRERAFDANT MTSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH

    PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL

    VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH

    VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVM RLRKLAQQIA

    NCKQCIERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI

    PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPTIREE LCTASYDTIT

    VHWTSDDEFS VVSYELQYTI FTGQANVVSL CNSADSWMIV PNIKQNHYTV

    HGLQSGTKYI FMVKAINQAG SRSSEPGKLK TNSQPFKLDP KSAHRKLKVS

    HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR HYWEVVISGS

    TWYAIGLAYK SAPKHEWIGK NSASWALCRC NNNWVVRHNS KEIPIEPAPH

    LRRVGILLDY DNGSIAFYDA LNSIHLYTFD VAFAQPVCPT FTVWNKCLTI

    ITGLPIPDHL DCTEQLP

  • UCSC uc004cte.5; human. [O15344-1];



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