Human ACAT1 ELISA Kit

Alternative name
Human acetoacetyl-CoA thiolase ELISA Kit;Human T2 ELISA Kit;Human ACAT ELISA Kit;Human MAT ELISA Kit;Human THIL ELISA Kit;Human acetyl-CoA acetyltransferase 1 ELISA Kit;Human acetyl-CoA acetyltransferase, mitochondrial ELISA Kit;Human acetoacetyl Coenzyme A thiolase ELISA Kit;Human acetyl-Coenzyme A acetyltransferase 1 ELISA Kit;Human mitochondrial acetoacetyl-CoA thiolase ELISA Kit;Human testicular tissue protein Li 198 ELISA Kit;
Catalog
E000245
species
Human
GeneACAT1
Other Species Mouse Acat1 ELISA Kit Bovine ACAT1 ELISA Kit Monkey ACAT1 ELISA Kit Rat Acat1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ACAT1. No significant cross-reactivity or interference between human ACAT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ACAT1 is typically less than 0.04 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman ACAT1 ELISA Kit allows for the in vitro quantitative determination of ACAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Acetyl-CoA acetyltransferase, mitochondrial Protein information
Uniprot ID THIL_HUMAN
Uniprot AC P24752; B2R6H1; G3XAB4; Q96FG8;
UniGene Hs.232375;
GeneID 38
KEGG hsa:38;
Human Acetyl-CoA acetyltransferase, mitochondrial Protein SEQUENCE
SEQUENCE 427 AA; 45200 MW; 2E81168EB39D0142 CRC64;
MAVLAALLRS GARSRSPLLR RLVQEIRYVE RSYVSKPTLK EVVIVSATRT
PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG
QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA
GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT
AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV
KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA
AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW
EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH
ALKQGEYGLA SICNGGGGAS AMLIQKL
UCSC uc001pjw.2; human. [P24752-1];

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