-
Alternative name
Human Autophagy-related protein LC3 A ELISA Kit;Human autophagy-related ubiquitin-like modifier LC3 A ELISA Kit;Human MAP1 light chain 3-like protein 1 ELISA Kit;Human MAP1A/MAP1B light chain 3 A ELISA Kit;Human MAP1A/MAP1B LC3 A ELISA Kit;Human Microtubule-associated protein 1 light chain 3 alpha ELISA Kit;Human ATG8E ELISA Kit;Human LC3 ELISA Kit;Human LC3A ELISA Kit;Human MAP1ALC3 ELISA Kit;Human MAP1BLC3 ELISA Kit;Human microtubule associated protein 1 light chain 3 alpha ELISA Kit;Human microtubule-associated proteins 1A/1B light chain 3A ELISA Kit;Human MAP1A/1B light chain 3 A ELISA Kit;Human microtubule-associated proteins 1A/1B light chain 3 ELISA Kit;
-
Catalog
E024483
-
species
Human
-
GeneMAP1LC3A
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a).
No significant cross-reactivity or interference between Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityTypically less than 0.62ng/mL.
-
Intended UseHuman MAP1LC3A ELISA Kit allows for the in vitro quantitative determination of MAP1LC3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Human Microtubule-associated proteins 1A/1B light chain 3A Protein information
-
Uniprot ID
MLP3A_HUMAN
-
Uniprot AC
Q9H492; E1P5P4; E1P5P5; Q9BXW5;
-
UniGene
Hs.632273;
-
GeneID
84557
-
KEGG
hsa:84557;
-
Human Microtubule-associated proteins 1A/1B light chain 3A Protein SEQUENCE
-
SEQUENCE 121 AA; 14272 MW; 48C1FBE8F7892AF3 CRC64;
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT
KFLVPDHVNM
SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE
QEKDEDGFLY MVYASQETFG
F
-
UCSC
uc002xap.3; human. [Q9H492-1];
-