Human ANXA6 ELISA Kit

Alternative name
Human 67 kDa calelectrin ELISA Kit;Human Annexin VI ELISA Kit;Human annexin-6 ELISA Kit;Human Calphobindin-II ELISA Kit;Human CPB-II ELISA Kit;Human chromobindin-20 ELISA Kit;Human lipocortin VI ELISA Kit;Human Protein III ELISA Kit;Human p68 ELISA Kit;Human p70 ELISA Kit;Human ANX6 ELISA Kit;Human CBP68 ELISA Kit;Human annexin A6 ELISA Kit;Human annexin VI (p68) ELISA Kit;Human calcium-binding protein p68 ELISA Kit;Human calelectrin ELISA Kit;Human calphobindin II ELISA Kit;Human testis secretory sperm-binding protein Li 198a ELISA Kit;
Catalog
E002447
species
Human
GeneANXA6
Standard Curve
Other Species Mouse Anxa6 ELISA Kit Bovine ANXA6 ELISA Kit Chicken ANXA6 ELISA Kit Rabbit ANXA6 ELISA Kit Rat Anxa6 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ANX6. No significant cross-reactivity or interference between ANX6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANX6 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ANXA6 ELISA Kit allows for the in vitro quantitative determination of ANXA6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This ANX6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANX6. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ANX6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANX6 antibody and an ANX6-HRP conjugate. The assay sample and buffer are incubated together with ANX6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANX6 concentration since ANX6 from samples and ANX6-HRP conjugate compete for the anti-ANX6 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANX6 from the sample, fewer sites are left to bind ANX6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANX6 concentration in each sample is interpolated from this standard curve.
Human Annexin A6 Protein information
Uniprot ID ANXA6_HUMAN
Uniprot AC P08133; B7Z8A7; D3DQH4; E9PGK1; Q6ZT79;
UniGene Hs.412117;
GeneID 309
KEGG hsa:309;
Human Annexin A6 Protein SEQUENCE
SEQUENCE 673 AA; 75873 MW; 90F47474F7F6D7B6 CRC64;
MAKPAQGAKY RGSIHDFPGF DPNQDAEALY TAMKGFGSDK EAILDIITSR
SNRQRQEVCQ SYKSLYGKDL IADLKYELTG KFERLIVGLM RPPAYCDAKE
IKDAISGIGT DEKCLIEILA SRTNEQMHQL VAAYKDAYER DLEADIIGDT
SGHFQKMLVV LLQGTREEDD VVSEDLVQQD VQDLYEAGEL KWGTDEAQFI
YILGNRSKQH LRLVFDEYLK TTGKPIEASI RGELSGDFEK LMLAVVKCIR
STPEYFAERL FKAMKGLGTR DNTLIRIMVS RSELDMLDIR EIFRTKYEKS
LYSMIKNDTS GEYKKTLLKL SGGDDDAAGQ FFPEAAQVAY QMWELSAVAR
VELKGTVRPA NDFNPDADAK ALRKAMKGLG TDEDTIIDII THRSNVQRQQ
IRQTFKSHFG RDLMTDLKSE ISGDLARLIL GLMMPPAHYD AKQLKKAMEG
AGTDEKALIE ILATRTNAEI RAINEAYKED YHKSLEDALS SDTSGHFRRI
LISLATGHRE EGGENLDQAR EDAQVAAEIL EIADTPSGDK TSLETRFMTI
LCTRSYPHLR RVFQEFIKMT NYDVEHTIKK EMSGDVRDAF VAIVQSVKNK
PLFFADKLYK SMKGAGTDEK TLTRIMVSRS EIDLLNIRRE FIEKYDKSLH
QAIEGDTSGD FLKALLALCG GED
UCSC uc003ltl.3; human. [P08133-1];

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Human ANXA6 ELISA Kit