Human MFAP4 ELISA Kit

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  • Alternative name

    Human microfibrillar associated protein 4 ELISA Kit;Human microfibril-associated glycoprotein 4 ELISA Kit;

  • Catalog
    E024449
  • species
    Human
  • GeneMFAP4
  • Standard CurveHuman MFAP4 ELISA Kit
  • Other Species Mouse Mfap4 ELISA KitBovine MFAP4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Microfibrillar Associated Protein 4 (MFAP4). No significant cross-reactivity or interference between Microfibrillar Associated Protein 4 (MFAP4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.125ng/mL.
  • Intended UseHuman MFAP4 ELISA Kit allows for the in vitro quantitative determination of MFAP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microfibrillar Associated Protein 4 (MFAP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microfibrillar Associated Protein 4 (MFAP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microfibrillar Associated Protein 4 (MFAP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microfibrillar Associated Protein 4 (MFAP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Microfibril-associated glycoprotein 4 Protein information
  • Uniprot ID MFAP4_HUMAN
  • Uniprot AC P55083; A8KAJ1; A8MVM2; B4E317; Q6P680;
  • UniGene Hs.296049;
  • GeneID 4239
  • KEGG hsa:4239;
  • Human Microfibril-associated glycoprotein 4 Protein SEQUENCE
  • SEQUENCE 255 AA; 28648 MW; B8F0B47AC694435E CRC64;

    MKALLALPLL LLLSTPPCAP QVSGIRGDAL ERFCLQQPLD CDDIYAQGYQ

    SDGVYLIYPS GPSVPVPVFC DMTTEGGKWT VFQKRFNGSV SFFRGWNDYK

    LGFGRADGEY WLGLQNMHLL TLKQKYELRV DLEDFENNTA YAKYADFSIS

    PNAVSAEEDG YTLFVAGFED GGAGDSLSYH SGQKFSTFDR DQDLFVQNCA

    ALSSGAFWFR SCHFANLNGF YLGGSHLSYA NGINWAQWKG FYYSLKRTEM

    KIRRA

  • UCSC uc002gvs.4; human. [P55083-1];


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