Human MTAP ELISA Kit

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  • Alternative name

    Human 5'-methylthioadenosine phosphorylase ELISA Kit;Human MTA phosphorylase ELISA Kit;Human MTAP ELISA Kit;Human MTAPase ELISA Kit;Human MSAP ELISA Kit;Human BDMF ELISA Kit;Human DMSFH ELISA Kit;Human DMSMFH ELISA Kit;Human HEL-249 ELISA Kit;Human LGMBF ELISA Kit;Human c86fus ELISA Kit;Human methylthioadenosine phosphorylase ELISA Kit;Human S-methyl-5'-thioadenosine phosphorylase ELISA Kit;Human MeSAdo phosphorylase ELISA Kit;Human epididymis luminal protein 249 ELISA Kit;

  • Catalog
    E024380
  • species
    Human
  • GeneMTAP
  • Standard CurveHuman MTAP ELISA Kit
  • Other Species Mouse Mtap ELISA KitBovine MTAP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Methylthioadenosine Phosphorylase (MTAP). No significant cross-reactivity or interference between Methylthioadenosine Phosphorylase (MTAP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman MTAP ELISA Kit allows for the in vitro quantitative determination of MTAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylthioadenosine Phosphorylase (MTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylthioadenosine Phosphorylase (MTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylthioadenosine Phosphorylase (MTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylthioadenosine Phosphorylase (MTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human S-methyl-5'-thioadenosine phosphorylase Protein information
  • Uniprot ID MTAP_HUMAN
  • Uniprot AC Q9H010;
  • UniGene Hs.193268;
  • GeneID 4507
  • KEGG hsa:4507;
  • Human S-methyl-5'-thioadenosine phosphorylase Protein SEQUENCE
  • SEQUENCE 283 AA; 31236 MW; 3B34C565EB5B99DA CRC64;

    MASGTTTTAV KIGIIGGTGL DDPEILEGRT EKYVDTPFGK PSDALILGKI

    KNVDCVLLAR HGRQHTIMPS KVNYQANIWA LKEEGCTHVI VTTACGSLRE

    EIQPGDIVII DQFIDRTTMR PQSFYDGSHS CARGVCHIPM AEPFCPKTRE

    VLIETAKKLG LRCHSKGTMV TIEGPRFSSR AESFMFRTWG ADVINMTTVP

    EVVLAKEAGI CYASIAMATD YDCWKEHEEA VSVDRVLKTL KENANKAKSL

    LLTTIPQIGS TEWSETLHNL KNMAQFSVLL PRH

  • UCSC uc003zph.4; human. [Q13126-1];


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