Human MTHFR ELISA Kit

Contact Us
  • Alternative name

    Human methylenetetrahydrofolate reductase ELISA Kit;Human 5,10-methylenetetrahydrofolate reductase (NADPH) ELISA Kit;Human methylenetetrahydrofolate reductase (NAD(P)H) ELISA Kit;

  • Catalog
    E024370
  • species
    Human
  • GeneMTHFR
  • Standard CurveHuman MTHFR ELISA Kit
  • Other Species Mouse Mthfr ELISA KitBovine MTHFR ELISA KitMonkey MTHFR ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Methylenetetrahydrofolate Reductase (MTHFR). No significant cross-reactivity or interference between Methylenetetrahydrofolate Reductase (MTHFR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 27.9pg/mL.
  • Intended UseHuman MTHFR ELISA Kit allows for the in vitro quantitative determination of MTHFR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylenetetrahydrofolate Reductase (MTHFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylenetetrahydrofolate Reductase (MTHFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Methylenetetrahydrofolate reductase Protein information
  • Uniprot ID MTHR_HUMAN
  • Uniprot AC P42898; B2R7A6; Q5SNW6; Q5SNW9; Q7Z6M6; Q8IU73; Q9UQR2;
  • UniGene Hs.214142; Hs.737916;
  • GeneID 4524
  • KEGG hsa:4524;
  • Human Methylenetetrahydrofolate reductase Protein SEQUENCE
  • SEQUENCE 656 AA; 74597 MW; F16E774833D054B8 CRC64;

    MVNEARGNSS LNPCLEGSAS SGSESSKDSS RCSTPGLDPE RHERLREKMR

    RRLESGDKWF SLEFFPPRTA EGAVNLISRF DRMAAGGPLY IDVTWHPAGD

    PGSDKETSSM MIASTAVNYC GLETILHMTC CRQRLEEITG HLHKAKQLGL

    KNIMALRGDP IGDQWEEEEG GFNYAVDLVK HIRSEFGDYF DICVAGYPKG

    HPEAGSFEAD LKHLKEKVSA GADFIITQLF FEADTFFRFV KACTDMGITC

    PIVPGIFPIQ GYHSLRQLVK LSKLEVPQEI KDVIEPIKDN DAAIRNYGIE

    LAVSLCQELL ASGLVPGLHF YTLNREMATT EVLKRLGMWT EDPRRPLPWA

    LSAHPKRREE DVRPIFWASR PKSYIYRTQE WDEFPNGRWG NSSSPAFGEL

    KDYYLFYLKS KSPKEELLKM WGEELTSEES VFEVFVLYLS GEPNRNGHKV

    TCLPWNDEPL AAETSLLKEE LLRVNRQGIL TINSQPNING KPSSDPIVGW

    GPSGGYVFQK AYLEFFTSRE TAEALLQVLK KYELRVNYHL VNVKGENITN

    APELQPNAVT WGIFPGREII QPTVVDPVSF MFWKDEAFAL WIERWGKLYE

    EESPSRTIIQ YIHDNYFLVN LVDNDFPLDN CLWQVVEDTL ELLNRPTQNA

    RETEAP

  • UCSC uc001atc.3; human. [P42898-1];



Inquiry About Human MTHFR ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!