Human MECP2 ELISA Kit

Alternative name
Human meCp-2 protein ELISA Kit;Human MeCp2 ELISA Kit;Human AUTSX3 ELISA Kit;Human MRX16 ELISA Kit;Human MRX79 ELISA Kit;Human MRXS13 ELISA Kit;Human MRXSL ELISA Kit;Human PPMX ELISA Kit;Human RS ELISA Kit;Human RTS ELISA Kit;Human RTT ELISA Kit;Human methyl-CpG binding protein 2 ELISA Kit;Human methyl-CpG-binding protein 2 ELISA Kit;Human testis tissue sperm-binding protein Li 41a ELISA Kit;
Catalog
E024349
species
Human
GeneMECP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Methyl CpG Binding Protein 2 (MECP2). No significant cross-reactivity or interference between Methyl CpG Binding Protein 2 (MECP2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman MECP2 ELISA Kit allows for the in vitro quantitative determination of MECP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methyl CpG Binding Protein 2 (MECP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methyl CpG Binding Protein 2 (MECP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methyl CpG Binding Protein 2 (MECP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methyl CpG Binding Protein 2 (MECP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Methyl-CpG-binding protein 2 Protein information
Uniprot ID MECP2_HUMAN
Uniprot AC P51608; O15233; Q6QHH9; Q7Z384;
UniGene Hs.200716; Hs.702514;
GeneID 4204
KEGG hsa:4204;
Human Methyl-CpG-binding protein 2 Protein SEQUENCE
SEQUENCE 486 AA; 52441 MW; EB6A33233AEDA566 CRC64;
MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA
HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL
PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL
DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK APGTGRGRGR PKGSGTTRPK
AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT TSTQVMVIKR
PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV
LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS
PKGRSSSASS PPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT
SPPEPQDLSS SVCKEEKMPR GGSLESDGCP KEPAKTQPAV ATAATAAEKY
KHRGEGERKD IVSSSMPRPN REEPVDSRTP VTERVS
UCSC uc004fjv.3; human. [P51608-1];

Category

Human MECP2 ELISA Kit