Human MTR ELISA Kit

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  • Alternative name

    Human 5-methyltetrahydrofolate--homocysteine methyltransferase ELISA Kit;Human vitamin-B12 dependent methionine synthase ELISA Kit;Human MS ELISA Kit;Human HMAG ELISA Kit;Human cblG ELISA Kit;Human 5-methyltetrahydrofolate-homocysteine methyltransferase ELISA Kit;Human methionine synthase ELISA Kit;Human 5-methyltetrahydrofolate-homocysteine methyltransferase 1 ELISA Kit;Human cobalamin-dependent methionine synthase ELISA Kit;

  • Catalog
    E024341
  • species
    Human
  • GeneMTR
  • Standard CurveHuman MTR ELISA Kit
  • Other Species Mouse Mtr ELISA KitBovine MTR ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MTR . No significant cross-reactivity or interference between MTR and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.375ng/ml
  • Intended UseHuman MTR ELISA Kit allows for the in vitro quantitative determination of MTR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MTR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MTR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTR can be calculated.
  • Human Methionine synthase Protein information
  • Uniprot ID METH_HUMAN
  • Uniprot AC Q99707; A1L4N8; A9Z1W4; B7ZLW7; B9EGF7; Q99713; Q99723;
  • UniGene Hs.498187;
  • GeneID 4548
  • KEGG hsa:4548;
  • Human Methionine synthase Protein SEQUENCE
  • SEQUENCE 1265 AA; 140527 MW; B04C26BCBE9A57C2 CRC64;

    MSPALQDLSQ PEGLKKTLRD EINAILQKRI MVLDGGMGTM IQREKLNEEH

    FRGQEFKDHA RPLKGNNDIL SITQPDVIYQ IHKEYLLAGA DIIETNTFSS

    TSIAQADYGL EHLAYRMNMC SAGVARKAAE EVTLQTGIKR FVAGALGPTN

    KTLSVSPSVE RPDYRNITFD ELVEAYQEQA KGLLDGGVDI LLIETIFDTA

    NAKAALFALQ NLFEEKYAPR PIFISGTIVD KSGRTLSGQT GEGFVISVSH

    GEPLCIGLNC ALGAAEMRPF IEIIGKCTTA YVLCYPNAGL PNTFGDYDET

    PSMMAKHLKD FAMDGLVNIV GGCCGSTPDH IREIAEAVKN CKPRVPPATA

    FEGHMLLSGL EPFRIGPYTN FVNIGERCNV AGSRKFAKLI MAGNYEEALC

    VAKVQVEMGA QVLDVNMDDG MLDGPSAMTR FCNLIASEPD IAKVPLCIDS

    SNFAVIEAGL KCCQGKCIVN SISLKEGEDD FLEKARKIKK YGAAMVVMAF

    DEEGQATETD TKIRVCTRAY HLLVKKLGFN PNDIIFDPNI LTIGTGMEEH

    NLYAINFIHA TKVIKETLPG ARISGGLSNL SFSFRGMEAI REAMHGVFLY

    HAIKSGMDMG IVNAGNLPVY DDIHKELLQL CEDLIWNKDP EATEKLLRYA

    QTQGTGGKKV IQTDEWRNGP VEERLEYALV KGIEKHIIED TEEARLNQKK

    YPRPLNIIEG PLMNGMKIVG DLFGAGKMFL PQVIKSARVM KKAVGHLIPF

    MEKEREETRV LNGTVEEEDP YQGTIVLATV KGDVHDIGKN IVGVVLGCNN

    FRVIDLGVMT PCDKILKAAL DHKADIIGLS GLITPSLDEM IFVAKEMERL

    AIRIPLLIGG ATTSKTHTAV KIAPRYSAPV IHVLDASKSV VVCSQLLDEN

    LKDEYFEEIM EEYEDIRQDH YESLKERRYL PLSQARKSGF QMDWLSEPHP

    VKPTFIGTQV FEDYDLQKLV DYIDWKPFFD VWQLRGKYPN RGFPKIFNDK

    TVGGEARKVY DDAHNMLNTL ISQKKLRARG VVGFWPAQSI QDDIHLYAEA

    AVPQAAEPIA TFYGLRQQAE KDSASTEPYY CLSDFIAPLH SGIRDYLGLF

    AVACFGVEEL SKAYEDDGDD YSSIMVKALG DRLAEAFAEE LHERVRRELW

    AYCGSEQLDV ADLRRLRYKG IRPAPGYPSQ PDHTEKLTMW RLADIEQSTG

    IRLTESLAMA PASAVSGLYF SNLKSKYFAV GKISKDQVED YALRKNISVA

    EVEKWLGPIL GYDTD

  • UCSC uc001hyi.5; human. [Q99707-1];


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