Human MT ELISA Kit

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  • Alternative name

    Metallothionein ELISA KIT; MT ELISA KIT; MT ELISA KIT

  • Catalog
    E024254
  • species
    Human
  • GeneMT
  • Standard CurveHuman MT ELISA Kit
  • Other Species Human MT ATP8 ELISA KitHuman MT ATP6 ELISA KitHuman MT CYB ELISA KitHuman MT CO2 ELISA KitHuman MT-CO3 ELISA KitHuman MT CO3 ELISA KitHuman MT ELISA KitHuman MT 1 ELISA KitHuman MT 3 ELISA KitHuman MT-ATP6 ELISA KitHuman MT-ATP8 ELISA KitHuman MT-CYB ELISA KitHuman MT-CO1 ELISA KitHuman MT-ND2 ELISA KitHuman MT-ND6 ELISA KitHuman Mt p53 ELISA KitHuman MT-P53 ELISA KitHuman MT-IgG ELISA KitHuman MT-IgM ELISA KitHuman MT ND1 ELISA KitHuman MT ND2 ELISA KitHuman MT ND3 ELISA KitHuman MT ND4 ELISA KitHuman MT ND4L ELISA KitHuman MT ND5 ELISA KitHuman MT ND6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MT. No significant cross-reactivity or interference between MT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MT ELISA Kit allows for the in vitro quantitative determination of MT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyMicrobiology
  • Product Description
    specifical
    Principle of the assay: MT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MT. Standards or samples are then added to the microtiter plate wells and MT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MT are added to each well to "sandwich" the MT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.



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