Human GRM1 ELISA Kit

Alternative name
Human MGLUR1 ELISA Kit;Human GPRC1A ELISA Kit;Human MGLU1 ELISA Kit;Human PPP1R85 ELISA Kit;Human SCAR13 ELISA Kit;Human glutamate metabotropic receptor 1 ELISA Kit;Human metabotropic glutamate receptor 1 ELISA Kit;Human glutamate receptor, metabotropic 1 ELISA Kit;Human protein phosphatase 1, regulatory subunit 85 ELISA Kit;
Catalog
E024211
species
Human
GeneGRM1
SpecificityThis assay has high sensitivity and excellent specificity for detection of GRM1. No significant cross-reactivity or interference between GRM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRM1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GRM1 ELISA Kit allows for the in vitro quantitative determination of GRM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: GRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRM1 antibody and an GRM1-HRP conjugate. The assay sample and buffer are incubated together with GRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRM1 concentration since GRM1 from samples and GRM1-HRP conjugate compete for the anti-GRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRM1 from the sample, fewer sites are left to bind GRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRM1 concentration in each sample is interpolated from this standard curve.
Human Metabotropic glutamate receptor 1 Protein information
Uniprot ID GRM1_HUMAN
Uniprot AC Q9NU10; Q9UGS9; Q9UGT0;
UniGene Hs.32945;
GeneID 2911
KEGG hsa:2911;
Human Metabotropic glutamate receptor 1 Protein SEQUENCE
SEQUENCE 1194 AA; 132357 MW; 0A633505F6D51909 CRC64;
MVGLLLFFFP AIFLEVSLLP RSPGRKVLLA GASSQRSVAR MDGDVIIGAL
FSVHHQPPAE KVPERKCGEI REQYGIQRVE AMFHTLDKIN ADPVLLPNIT
LGSEIRDSCW HSSVALEQSI EFIRDSLISI RDEKDGINRC LPDGQSLPPG
RTKKPIAGVI GPGSSSVAIQ VQNLLQLFDI PQIAYSATSI DLSDKTLYKY
FLRVVPSDTL QARAMLDIVK RYNWTYVSAV HTEGNYGESG MDAFKELAAQ
EGLCIAHSDK IYSNAGEKSF DRLLRKLRER LPKARVVVCF CEGMTVRGLL
SAMRRLGVVG EFSLIGSDGW ADRDEVIEGY EVEANGGITI KLQSPEVRSF
DDYFLKLRLD TNTRNPWFPE FWQHRFQCRL PGHLLENPNF KRICTGNESL
EENYVQDSKM GFVINAIYAM AHGLQNMHHA LCPGHVGLCD AMKPIDGSKL
LDFLIKSSFI GVSGEEVWFD EKGDAPGRYD IMNLQYTEAN RYDYVHVGTW
HEGVLNIDDY KIQMNKSGVV RSVCSEPCLK GQIKVIRKGE VSCCWICTAC
KENEYVQDEF TCKACDLGWW PNADLTGCEP IPVRYLEWSN IESIIAIAFS
CLGILVTLFV TLIFVLYRDT PVVKSSSREL CYIILAGIFL GYVCPFTLIA
KPTTTSCYLQ RLLVGLSSAM CYSALVTKTN RIARILAGSK KKICTRKPRF
MSAWAQVIIA SILISVQLTL VVTLIIMEPP MPILSYPSIK EVYLICNTSN
LGVVAPLGYN GLLIMSCTYY AFKTRNVPAN FNEAKYIAFT MYTTCIIWLA
FVPIYFGSNY KIITTCFAVS LSVTVALGCM FTPKMYIIIA KPERNVRSAF
TTSDVVRMHV GDGKLPCRSN TFLNIFRRKK AGAGNANSNG KSVSWSEPGG
GQVPKGQHMW HRLSVHVKTN ETACNQTAVI KPLTKSYQGS GKSLTFSDTS
TKTLYNVEEE EDAQPIRFSP PGSPSMVVHR RVPSAATTPP LPSHLTAEET
PLFLAEPALP KGLPPPLQQQ QQPPPQQKSL MDQLQGVVSN FSTAIPDFHA
VLAGPGGPGN GLRSLYPPPP PPQHLQMLPL QLSTFGEELV SPPADDDDDS
ERFKLLQEYV YEHEREGNTE EDELEEEEED LQAASKLTPD DSPALTPPSP
FRDSVASGSS VPSSPVSESV LCTPPNVSYA SVILRDYKQS SSTL
UCSC uc003qll.4; human. [Q13255-1];

Category

Human GRM1 ELISA Kit