Human MSLN ELISA Kit

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  • Alternative name

    Human CAK1 antigen ELISA Kit;Human pre-pro-megakaryocyte-potentiating factor ELISA Kit;Human MPF ELISA Kit;Human SMRP ELISA Kit;Human mesothelin ELISA Kit;Human megakaryocyte potentiating factor ELISA Kit;Human soluble MPF mesothelin related protein ELISA Kit;

  • Catalog
    E024202
  • species
    Human
  • GeneMSLN
  • Other Species Mouse Msln ELISA KitRat Msln ELISA Kit
  • SpecificityThe Human Mesothelin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Mesothelin proteins within the range of 156 pg/ml - 10000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman MSLN ELISA Kit allows for the in vitro quantitative determination of MSLN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Human Mesothelin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Mesothelin in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human Mesothelin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mesothelin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Mesothelin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
  • Human Mesothelin, cleaved form Protein information
  • Uniprot ID MSLN_HUMAN
  • Uniprot AC PSGKKARE IDESLIFYKK WELEACVDAA LLATQMDRVN AIPFTYEQLD VLKHKLDELY
  • UniGene Hs.408488;
  • GeneID 10232
  • KEGG hsa:10232;
  • Human Mesothelin, cleaved form Protein SEQUENCE
  • SEQUENCE 630 AA; 68986 MW; FA17E3609B6CC9CA CRC64;

    MALPTARPLL GSCGTPALGS LLFLLFSLGW VQPSRTLAGE TGQEAAPLDG

    VLANPPNISS LSPRQLLGFP CAEVSGLSTE RVRELAVALA QKNVKLSTEQ

    LRCLAHRLSE PPEDLDALPL DLLLFLNPDA FSGPQACTRF FSRITKANVD

    LLPRGAPERQ RLLPAALACW GVRGSLLSEA DVRALGGLAC DLPGRFVAES

    AEVLLPRLVS CPGPLDQDQQ EAARAALQGG GPPYGPPSTW SVSTMDALRG

    LLPVLGQPII RSIPQGIVAA WRQRSSRDPS WRQPERTILR PRFRREVEKT

    ACPSGKKARE IDESLIFYKK WELEACVDAA LLATQMDRVN AIPFTYEQLD

    VLKHKLDELY PQGYPESVIQ HLGYLFLKMS PEDIRKWNVT SLETLKALLE

    VNKGHEMSPQ APRRPLPQVA TLIDRFVKGR GQLDKDTLDT LTAFYPGYLC

    SLSPEELSSV PPSSIWAVRP QDLDTCDPRQ LDVLYPKARL AFQNMNGSEY

    FVKIQSFLGG APTEDLKALS QQNVSMDLAT FMKLRTDAVL PLTVAEVQKL

    LGPHVEGLKA EERHRPVRDW ILRQRQDDLD TLGLGLQGGI PNGYLVLDLS

    MQEALSGTPC LLGPGPVLTV LALLLASTLA

  • UCSC uc002cjt.2; human. [Q13421-1];


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