Human MANF ELISA Kit

Alternative name
Human Arginine-rich protein ELISA Kit;Human Protein ARMET ELISA Kit;Human ARMET ELISA Kit;Human ARP ELISA Kit;Human mesencephalic astrocyte derived neurotrophic factor ELISA Kit;Human mesencephalic astrocyte-derived neurotrophic factor ELISA Kit;Human arginine-rich, mutated in early stage tumors ELISA Kit;
Catalog
E024187
species
Human
GeneMANF
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). No significant cross-reactivity or interference between Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 6.7pg/mL.
Intended UseHuman MANF ELISA Kit allows for the in vitro quantitative determination of MANF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Mesencephalic astrocyte-derived neurotrophic factor Protein information
Uniprot ID MANF_HUMAN
Uniprot AC P55145; Q14CX4; Q86U67; Q96IS4;
UniGene Hs.436446;
GeneID 7873
KEGG hsa:7873;
Human Mesencephalic astrocyte-derived neurotrophic factor Protein SEQUENCE
SEQUENCE 182 AA; 20700 MW; EE25A5D1A58D1AD7 CRC64;
MRRMWATQGL AVALALSVLP GSRALRPGDC EVCISYLGRF YQDLKDRDVT
FSPATIENEL IKFCREARGK ENRLCYYIGA TDDAATKIIN EVSKPLAHHI
PVEKICEKLK KKDSQICELK YDKQIDLSTV DLKKLRVKEL KKILDDWGET
CKGCAEKSDY IRKINELMPK YAPKAASART DL
UCSC uc003dbc.4; human.;

Human MANF ELISA Kit

Contact Us