Human MTNR1A ELISA Kit

Alternative name
Human MEL-1A-R ELISA Kit;Human mel1a receptor ELISA Kit;Human MT1 ELISA Kit;Human melatonin receptor 1A ELISA Kit;Human melatonin receptor type 1A ELISA Kit;
Catalog
E024086
species
Human
GeneMTNR1A
Standard Curve
Other Species Mouse Mtnr1a ELISA Kit Bovine MTNR1A ELISA Kit Porcine MTNR1A ELISA Kit Rat Mtnr1a ELISA Kit Sheep MTNR1A ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of MTNR-1A. No significant cross-reactivity or interference between MTNR-1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MTNR-1A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman MTNR1A ELISA Kit allows for the in vitro quantitative determination of MTNR1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This MTNR-1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MTNR-1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||MTNR-1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MTNR-1A antibody and an MTNR-1A-HRP conjugate. The assay sample and buffer are incubated together with MTNR-1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTNR-1A concentration since MTNR-1A from samples and MTNR-1A-HRP conjugate compete for the anti-MTNR-1A antibody binding site. Since the number of sites is limited, as more sites are occupied by MTNR-1A from the sample, fewer sites are left to bind MTNR-1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTNR-1A concentration in each sample is interpolated from this standard curve.
Human Melatonin receptor type 1A Protein information
Uniprot ID MTR1A_HUMAN
Uniprot AC P48039; A0AVC5; B0M0L2;
UniGene Hs.243467;
GeneID 4543
KEGG hsa:4543;
Human Melatonin receptor type 1A Protein SEQUENCE
SEQUENCE 350 AA; 39375 MW; DF7C67111D2386ED CRC64;
MQGNGSALPN ASQPVLRGDG ARPSWLASAL ACVLIFTIVV DILGNLLVIL
SVYRNKKLRN AGNIFVVSLA VADLVVAIYP YPLVLMSIFN NGWNLGYLHC
QVSGFLMGLS VIGSIFNITG IAINRYCYIC HSLKYDKLYS SKNSLCYVLL
IWLLTLAAVL PNLRAGTLQY DPRIYSCTFA QSVSSAYTIA VVVFHFLVPM
IIVIFCYLRI WILVLQVRQR VKPDRKPKLK PQDFRNFVTM FVVFVLFAIC
WAPLNFIGLA VASDPASMVP RIPEWLFVAS YYMAYFNSCL NAIIYGLLNQ
NFRKEYRRII VSLCTARVFF VDSSNDVADR VKWKPSPLMT NNNVVKVDSV
UCSC uc003izd.2; human.;

Category

Human MTNR1A ELISA Kit

Human MEL-1A-R ELISA Kit;Human mel1a receptor ELISA Kit;Human MT1 ELISA Kit;Human melatonin receptor 1A ELISA Kit;Human melatonin receptor type 1A ELISA Kit;