Human MIA ELISA Kit

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  • Alternative name

    Human Melanoma inhibitory activity protein ELISA Kit;Human CD-RAP ELISA Kit;Human melanoma inhibitory activity ELISA Kit;Human melanoma-derived growth regulatory protein ELISA Kit;

  • Catalog
    E024052
  • species
    Human
  • GeneMIA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MIA. No significant cross-reactivity or interference between Human MIA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.195 ng/ml
  • Intended UseHuman MIA ELISA Kit allows for the in vitro quantitative determination of MIA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIA bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Melanoma-derived growth regulatory protein Protein information
  • Uniprot ID MIA_HUMAN
  • Uniprot AC Q16674; Q6FHV3;
  • UniGene Hs.646364;
  • GeneID 8190
  • KEGG hsa:8190;
  • Human Melanoma-derived growth regulatory protein Protein SEQUENCE
  • SEQUENCE 131 AA; 14509 MW; 4D3BB30BD6008BDC CRC64;

    MARSLVCLGV IILLSAFSGP GVRGGPMPKL ADRKLCADQE CSHPISMAVA

    LQDYMAPDCR FLTIHRGQVV YVFSKLKGRG RLFWGGSVQG DYYGDLAARL

    GYFPSSIVRE DQTLKPGKVD VKTDKWDFYC Q

  • UCSC uc002opb.5; human. [Q16674-1];



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