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Human MCAM ELISA Kit

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  • Alternative name

    Human cell surface glycoprotein P1H12 ELISA Kit;Human melanoma cell adhesion molecule ELISA Kit;Human melanoma-associated antigen A32 ELISA Kit;Human melanoma-associated antigen MUC18 ELISA Kit;Human S-endo 1 endothelial-associated antigen ELISA Kit;Human MUC18 ELISA Kit;Human CD146 ELISA Kit;Human cell surface glycoprotein MUC18 ELISA Kit;Human Gicerin ELISA Kit;Human melanoma adhesion molecule ELISA Kit;
  • Catalog
    E024047
  • species
    Human
  • GeneMCAM
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule (MCAM). No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule (MCAM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.67ng/mL.
  • Intended UseHuman MCAM ELISA Kit allows for the in vitro quantitative determination of MCAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Cell Adhesion Molecule (MCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Cell Adhesion Molecule (MCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cell surface glycoprotein MUC18 Protein information
  • Uniprot ID MUC18_HUMAN
  • Uniprot AC P43121; O95812; Q59E86; Q6PHR3; Q6ZTR2;
  • UniGene Hs.599039;
  • GeneID 4162
  • KEGG hsa:4162;
  • Human Cell surface glycoprotein MUC18 Protein SEQUENCE

  • SEQUENCE 646 AA; 71607 MW; E46CB8AC7BA0738E CRC64;

    MGLPRLVCAF LLAACCCCPR VAGVPGEAEQ PAPELVEVEV GSTALLKCGL

    SQSQGNLSHV DWFSVHKEKR TLIFRVRQGQ GQSEPGEYEQ RLSLQDRGAT

    LALTQVTPQD ERIFLCQGKR PRSQEYRIQL RVYKAPEEPN IQVNPLGIPV

    NSKEPEEVAT CVGRNGYPIP QVIWYKNGRP LKEEKNRVHI QSSQTVESSG

    LYTLQSILKA QLVKEDKDAQ FYCELNYRLP SGNHMKESRE VTVPVFYPTE

    KVWLEVEPVG MLKEGDRVEI RCLADGNPPP HFSISKQNPS TREAEEETTN

    DNGVLVLEPA RKEHSGRYEC QGLDLDTMIS LLSEPQELLV NYVSDVRVSP

    AAPERQEGSS LTLTCEAESS QDLEFQWLRE ETGQVLERGP VLQLHDLKRE

    AGGGYRCVAS VPSIPGLNRT QLVNVAIFGP PWMAFKERKV WVKENMVLNL

    SCEASGHPRP TISWNVNGTA SEQDQDPQRV LSTLNVLVTP ELLETGVECT

    ASNDLGKNTS ILFLELVNLT TLTPDSNTTT GLSTSTASPH TRANSTSTER

    KLPEPESRGV VIVAVIVCIL VLAVLGAVLY FLYKKGKLPC RRSGKQEITL

    PPSRKSELVV EVKSDKLPEE MGLLQGSSGD KRAPGDQGEK YIDLRH

  • UCSC uc001pwf.4; human. [P43121-1];



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