Human MAA1 ELISA Kit

Contact Us
  • Alternative name

    Melanoma-associated antigen 1 ELISA KIT; Antigen MZ2-E ELISA KIT; Cancer/testis antigen 1.1 ELISA KIT; CT1.1 ELISA KIT; MAGE-1 antigenMAGEA1 ELISA KIT; MAGE1 ELISA KIT; MAGE1A ELISA KIT; CT1.1 ELISA KIT

  • Catalog
    E024038
  • species
    Human
  • GeneMAA1
  • Standard CurveHuman MAA1 ELISA Kit
  • Other Species Mouse MAA1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman MAA1 ELISA Kit allows for the in vitro quantitative determination of MAA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Intended Uses: This MAGE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MAGE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MAGE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAGE1 antibody and an MAGE1-HRP conjugate. The assay sample and buffer are incubated together with MAGE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAGE1 concentration since MAGE1 from samples and MAGE1-HRP conjugate compete for the anti-MAGE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAGE1 from the sample, fewer sites are left to bind MAGE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAGE1 concentration in each sample is interpolated from this standard curve.



Inquiry About Human MAA1 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!