Human PMEL ELISA Kit

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  • Alternative name

    Human ME20-M ELISA Kit;Human ME20M ELISA Kit;Human melanocyte protein Pmel 17 ELISA Kit;Human melanocytes lineage-specific antigen GP100 ELISA Kit;Human melanoma-associated ME20 antigen ELISA Kit;Human P1 ELISA Kit;Human P100 ELISA Kit;Human premelanosome protein ELISA Kit;Human silver locus protein homolog ELISA Kit;Human 95 kDa melanocyte-specific secreted glycoprotein ELISA Kit;Human P26 ELISA Kit;Human Secreted melanoma-associated ME20 antigen ELISA Kit;Human ME20-S ELISA Kit;Human ME20S ELISA Kit;Human D12S53E ELISA Kit;Human PMEL17 ELISA Kit;Human SILV ELISA Kit;Human ME20 ELISA Kit;Human SI ELISA Kit;Human SIL ELISA Kit;Human gp100 ELISA Kit;Human melanocyte protein PMEL ELISA Kit;Human melanocyte protein mel 17 ELISA Kit;Human melanosomal matrix protein17 ELISA Kit;Human silver, mouse, homolog of ELISA Kit;

  • Catalog
    E023998
  • species
    Human
  • GenePMEL
  • Other Species Mouse Pmel ELISA KitBovine PMEL ELISA KitChicken PMEL ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman PMEL ELISA Kit allows for the in vitro quantitative determination of PMEL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PMEL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PMEL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PMEL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman PMEL can be calculated.
  • Human M-beta Protein information
  • Uniprot ID PMEL_HUMAN
  • Uniprot AC P40967; B3KS57; B7Z6D7; Q12763; Q14448; Q14817; Q16565;
  • UniGene Hs.95972;
  • GeneID 6490
  • KEGG hsa:6490;
  • Human M-beta Protein SEQUENCE
  • SEQUENCE 661 AA; 70255 MW; 8A904FAB16715653 CRC64;

    MDLVLKRCLL HLAVIGALLA VGATKVPRNQ DWLGVSRQLR TKAWNRQLYP

    EWTEAQRLDC WRGGQVSLKV SNDGPTLIGA NASFSIALNF PGSQKVLPDG

    QVIWVNNTII NGSQVWGGQP VYPQETDDAC IFPDGGPCPS GSWSQKRSFV

    YVWKTWGQYW QVLGGPVSGL SIGTGRAMLG THTMEVTVYH RRGSRSYVPL

    AHSSSAFTIT DQVPFSVSVS QLRALDGGNK HFLRNQPLTF ALQLHDPSGY

    LAEADLSYTW DFGDSSGTLI SRALVVTHTY LEPGPVTAQV VLQAAIPLTS

    CGSSPVPGTT DGHRPTAEAP NTTAGQVPTT EVVGTTPGQA PTAEPSGTTS

    VQVPTTEVIS TAPVQMPTAE STGMTPEKVP VSEVMGTTLA EMSTPEATGM

    TPAEVSIVVL SGTTAAQVTT TEWVETTARE LPIPEPEGPD ASSIMSTESI

    TGSLGPLLDG TATLRLVKRQ VPLDCVLYRY GSFSVTLDIV QGIESAEILQ

    AVPSGEGDAF ELTVSCQGGL PKEACMEISS PGCQPPAQRL CQPVLPSPAC

    QLVLHQILKG GSGTYCLNVS LADTNSLAVV STQLIMPGQE AGLGQVPLIV

    GILLVLMAVV LASLIYRRRL MKQDFSVPQL PHSSSHWLRL PRIFCSCPIG

    ENSPLLSGQQ V

  • UCSC uc001sip.4; human. [P40967-1];



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