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Catalog
E023966
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species
Human
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human melanin. No significant cross-reactivity or interference between human melanin and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 15.6 pg/ml.
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Intended UseHuman MLANA ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for melanin and Horseradish Peroxidase (HRP) conjugated melanin. The competitive inhibition reaction is launched between with HRP labeled melanin and unlabeled melanin with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of melanin in the sample. The color development is stopped and the intensity of the color is measured.
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