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Human OPN1MW ELISA Kit

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  • Alternative name

    Human green cone photoreceptor pigment ELISA Kit;Human green-sensitive opsin ELISA Kit;Human GOP ELISA Kit;Human GCP ELISA Kit;Human opsin 1, medium wave sensitive 2 ELISA Kit;Human medium-wave-sensitive opsin 1 ELISA Kit;Human opsin 1 (cone pigments), medium-wave-sensitive 2 ELISA Kit;Human opsin 1 cone pigments medium-wave-sensitive 2 ELISA Kit;
  • Catalog
    E023949
  • species
    Human
  • GeneOPN1MW
  • Standard CurveHuman OPN1MW ELISA Kit
  • Other Species Mouse Opn1mw ELISA KitGuinea Pig OPN1MW ELISA KitRabbit OPN1MW ELISA KitRat Opn1mw ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OPN1MW. No significant cross-reactivity or interference between OPN1MW and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPN1MW and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman OPN1MW ELISA Kit allows for the in vitro quantitative determination of OPN1MW , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: OPN1MW ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPN1MW antibody and an OPN1MW-HRP conjugate. The assay sample and buffer are incubated together with OPN1MW-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPN1MW concentration since OPN1MW from samples and OPN1MW-HRP conjugate compete for the anti-OPN1MW antibody binding site. Since the number of sites is limited, as more sites are occupied by OPN1MW from the sample, fewer sites are left to bind OPN1MW-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPN1MW concentration in each sample is interpolated from this standard curve.
  • Human Medium-wave-sensitive opsin 1 Protein information
  • Uniprot ID OPSG_HUMAN
  • Uniprot AC P04001;
  • UniGene Hs.247787; Hs.571751;
  • GeneID 728458
  • KEGG hsa:728458;
  • Human Medium-wave-sensitive opsin 1 Protein SEQUENCE

  • SEQUENCE 364 AA; 40584 MW; A98D046958C72AE9 CRC64;

    MAQQWSLQRL AGRHPQDSYE DSTQSSIFTY TNSNSTRGPF EGPNYHIAPR

    WVYHLTSVWM IFVVIASVFT NGLVLAATMK FKKLRHPLNW ILVNLAVADL

    AETVIASTIS VVNQVYGYFV LGHPMCVLEG YTVSLCGITG LWSLAIISWE

    RWMVVCKPFG NVRFDAKLAI VGIAFSWIWA AVWTAPPIFG WSRYWPHGLK

    TSCGPDVFSG SSYPGVQSYM IVLMVTCCIT PLSIIVLCYL QVWLAIRAVA

    KQQKESESTQ KAEKEVTRMV VVMVLAFCFC WGPYAFFACF AAANPGYPFH

    PLMAALPAFF AKSATIYNPV IYVFMNRQFR NCILQLFGKK VDDGSELSSA

    SKTEVSSVSS VSPA

  • UCSC uc004fkb.4; human.;



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