Human MED31 ELISA Kit

Alternative name
Human mediator complex subunit 31 ELISA Kit;Human mediator complex subunit SOH1 ELISA Kit;Human hSOH1 ELISA Kit;Human Soh1 ELISA Kit;Human 3110004H13Rik ELISA Kit;Human CGI-125 ELISA Kit;Human mediator of RNA polymerase II transcription subunit 31 ELISA Kit;Human mediator of RNA polymerase II transcription, subunit 31 homolog ELISA Kit;
Catalog
E023940
species
Human
GeneMED31
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.938ng/ml
Intended UseHuman MED31 ELISA Kit allows for the in vitro quantitative determination of MED31 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MED31 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MED31 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MED31 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MED31 can be calculated.
Human Mediator of RNA polymerase II transcription subunit 31 Protein information
Uniprot ID MED31_HUMAN
Uniprot AC Q9Y3C7; B2R4L9;
UniGene Hs.567493;
GeneID 51003
KEGG hsa:51003;
Human Mediator of RNA polymerase II transcription subunit 31 Protein SEQUENCE
SEQUENCE 131 AA; 15805 MW; 5DC672ADB994D015 CRC64;
MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN
YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELVN AQCAKFIDEQ
QILHWQHYSR KRMRLQQALA EQQQQNNTSG K
UCSC uc002gdg.5; human.;

Human MED31 ELISA Kit

Contact Us