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Human ANXA1 ELISA Kit

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  • Alternative name

    Human Annexin I ELISA Kit;Human annexin-1 ELISA Kit;Human calpactin II ELISA Kit;Human calpactin-2 ELISA Kit;Human chromobindin-9 ELISA Kit;Human Lipocortin I ELISA Kit;Human phospholipase A2 inhibitory protein ELISA Kit;Human p35 ELISA Kit;Human ANX1 ELISA Kit;Human LPC1 ELISA Kit;Human annexin A1 ELISA Kit;Human annexin I (lipocortin I) ELISA Kit;
  • Catalog
    E002390
  • species
    Human
  • GeneANXA1
  • Standard CurveHuman ANXA1 ELISA Kit
  • Other Species Mouse Anxa1 ELISA KitBovine ANXA1 ELISA KitHorse ANXA1 ELISA KitPorcine ANXA1 ELISA KitRabbit ANXA1 ELISA KitRat Anxa1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ANXA1. No significant cross-reactivity or interference between ANXA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ANXA1 ELISA Kit allows for the in vitro quantitative determination of ANXA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Intended Uses: This ANXA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANXA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ANXA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA1 antibody and an ANXA1-HRP conjugate. The assay sample and buffer are incubated together with ANXA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA1 concentration since ANXA1 from samples and ANXA1-HRP conjugate compete for the anti-ANXA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA1 from the sample, fewer sites are left to bind ANXA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA1 concentration in each sample is interpolated from this standard curve.
  • Human Annexin A1 Protein information
  • Uniprot ID ANXA1_HUMAN
  • Uniprot AC P04083;
  • UniGene Hs.494173;
  • GeneID 301
  • KEGG hsa:301;
  • Human Annexin A1 Protein SEQUENCE

  • SEQUENCE 346 AA; 38714 MW; 14B42E1FA4178EC0 CRC64;

    MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA

    LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL

    TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR

    DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD

    SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK

    VLDLELKGDI EKCLTAIVKC ATSKPAFFAE KLHQAMKGVG TRHKALIRIM

    VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN



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