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Human MBD1 ELISA Kit

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  • Alternative name

    Human CXXC-type zinc finger protein 3 ELISA Kit;Human Methyl-CpG-binding protein MBD1 ELISA Kit;Human protein containing methyl-CpG-binding domain 1 ELISA Kit;Human CXXC3 ELISA Kit;Human PCM1 ELISA Kit;Human RFT ELISA Kit;Human methyl-CpG binding domain protein 1 ELISA Kit;Human methyl-CpG-binding domain protein 1 ELISA Kit;Human methyl-CpG binding domain protein 1 isoform PCM1 ELISA Kit;Human the regulator of fibroblast growth factor 2 (FGF-2) transcription ELISA Kit;
  • Catalog
    E023855
  • species
    Human
  • GeneMBD1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MBD1. No significant cross-reactivity or interference between Human MBD1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human MBD1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseHuman MBD1 ELISA Kit allows for the in vitro quantitative determination of MBD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MBD1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MBD1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human MBD1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBD1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MBD1. You can calculate the concentration of Human MBD1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Methyl-CpG-binding domain protein 1 Protein information
  • Uniprot ID MBD1_HUMAN
  • Uniprot AC QVTEDCGA CSTCLLQLPH DVASGLFCKC ERRRCLRIVE RSRGCGVCRG CQTQEDCGHC
  • UniGene Hs.405610;
  • GeneID 4152
  • KEGG hsa:4152;
  • Human Methyl-CpG-binding domain protein 1 Protein SEQUENCE

  • SEQUENCE 605 AA; 66607 MW; 665732782CC6A32A CRC64;

    MAEDWLDCPA LGPGWKRREV FRKSGATCGR SDTYYQSPTG DRIRSKVELT

    RYLGPACDLT LFDFKQGILC YPAPKAHPVA VASKKRKKPS RPAKTRKRQV

    GPQSGEVRKE APRDETKADT DTAPASFPAP GCCENCGISF SGDGTQRQRL

    KTLCKDCRAQ RIAFNREQRM FKRVGCGECA ACQVTEDCGA CSTCLLQLPH

    DVASGLFCKC ERRRCLRIVE RSRGCGVCRG CQTQEDCGHC PICLRPPRPG

    LRRQWKCVQR RCLRGKHARR KGGCDSKMAA RRRPGAQPLP PPPPSQSPEP

    TEPHPRALAP SPPAEFIYYC VDEDELQPYT NRRQNRKCGA CAACLRRMDC

    GRCDFCCDKP KFGGSNQKRQ KCRWRQCLQF AMKRLLPSVW SESEDGAGSP

    PPYRRRKRPS SARRHHLGPT LKPTLATRTA QPDHTQAPTK QEAGGGFVLP

    PPGTDLVFLR EGASSPVQVP GPVAASTEAL LQEAQCSGLS WVVALPQVKQ

    EKADTQDEWT PGTAVLTSPV LVPGCPSKAV DPGLPSVKQE PPDPEEDKEE

    NKDDSASKLA PEEEAGGAGT PVITEIFSLG GTRFRDTAVW LPRSKDLKKP

    GARKQ

  • UCSC uc002leg.4; human. [Q9UIS9-1];



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