Contact Us
  • Catalog
  • species
  • GeneMPF
  • Standard CurveHuman MPF ELISA Kit
  • Other Species Human MF/MPF ELISA KitMouse MPF ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MPF ELISA Kit allows for the in vitro quantitative determination of MPF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: MPF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MPF antibody and an MPF-HRP conjugate. The assay sample and buffer are incubated together with MPF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MPF concentration since MPF from samples and MPF-HRP conjugate compete for the anti-MPF antibody binding site. Since the number of sites is limited, as more sites are occupied by MPF from the sample, fewer sites are left to bind MPF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPF concentration in each sample is interpolated from this standard curve.

Related products with Human MPF ELISA Kit

Inquiry About Human MPF ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!