Human MMP28 ELISA Kit

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  • Alternative name

    Human MMP-28 ELISA Kit;Human EPILYSIN ELISA Kit;Human MMP25 ELISA Kit;Human MM28 ELISA Kit;Human MMP-25 ELISA Kit;Human matrix metallopeptidase 28 ELISA Kit;Human matrix metalloproteinase-28 ELISA Kit;Human matrix metalloprotease MMP25 ELISA Kit;

  • Catalog
    E023798
  • species
    Human
  • GeneMMP28
  • Standard CurveHuman MMP28 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 28 (MMP28). No significant cross-reactivity or interference between Matrix Metalloproteinase 28 (MMP28) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman MMP28 ELISA Kit allows for the in vitro quantitative determination of MMP28 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 28 (MMP28). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 28 (MMP28). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 28 (MMP28), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 28 (MMP28) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Matrix metalloproteinase-28 Protein information
  • Uniprot ID MMP28_HUMAN
  • Uniprot AC Q9H239; Q96F04; Q96TE2;
  • UniGene Hs.380710;
  • GeneID 79148
  • KEGG hsa:79148;
  • Human Matrix metalloproteinase-28 Protein SEQUENCE
  • SEQUENCE 520 AA; 58939 MW; E85D7ADA3069B063 CRC64;

    MVARVGLLLR ALQLLLWGHL DAQPAERGGQ ELRKEAEAFL EKYGYLNEQV

    PKAPTSTRFS DAIRAFQWVS QLPVSGVLDR ATLRQMTRPR CGVTDTNSYA

    AWAERISDLF ARHRTKMRRK KRFAKQGNKW YKQHLSYRLV NWPEHLPEPA

    VRGAVRAAFQ LWSNVSALEF WEAPATGPAD IRLTFFQGDH NDGLGNAFDG

    PGGALAHAFL PRRGEAHFDQ DERWSLSRRR GRNLFVVLAH EIGHTLGLTH

    SPAPRALMAP YYKRLGRDAL LSWDDVLAVQ SLYGKPLGGS VAVQLPGKLF

    TDFETWDSYS PQGRRPETQG PKYCHSSFDA ITVDRQQQLY IFKGSHFWEV

    AADGNVSEPR PLQERWVGLP PNIEAAAVSL NDGDFYFFKG GRCWRFRGPK

    PVWGLPQLCR AGGLPRHPDA ALFFPPLRRL ILFKGARYYV LARGGLQVEP

    YYPRSLQDWG GIPEEVSGAL PRPDGSIIFF RDDRYWRLDQ AKLQATTSGR

    WATELPWMGC WHANSGSALF

  • UCSC uc002hka.4; human. [Q9H239-1];



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