Human MMP19 ELISA Kit

Alternative name
Human MMP-19 ELISA Kit;Human matrix metalloproteinase RASI ELISA Kit;Human matrix metalloproteinase-18 ELISA Kit;Human MMP-18 ELISA Kit;Human MMP18 ELISA Kit;Human RASI ELISA Kit;Human CODA ELISA Kit;Human RASI-1 ELISA Kit;Human matrix metallopeptidase 19 ELISA Kit;Human matrix metalloproteinase-19 ELISA Kit;Human matrix metalloproteinase-beta19 ELISA Kit;
Catalog
E023782
species
Human
GeneMMP19
SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 19 (MMP19). No significant cross-reactivity or interference between Matrix Metalloproteinase 19 (MMP19) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.113ng/mL.
Intended UseHuman MMP19 ELISA Kit allows for the in vitro quantitative determination of MMP19 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 19 (MMP19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 19 (MMP19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 19 (MMP19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 19 (MMP19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Matrix metalloproteinase-19 Protein information
Uniprot ID MMP19_HUMAN
Uniprot AC Q99542; B4E030; O15278; O95606; Q99580;
UniGene Hs.591033;
GeneID 4327
KEGG hsa:4327;
Human Matrix metalloproteinase-19 Protein SEQUENCE
SEQUENCE 508 AA; 57357 MW; BA480549AA9A8972 CRC64;
MNCQQLWLGF LLPMTVSGRV LGLAEVAPVD YLSQYGYLQK PLEGSNNFKP
EDITEALRAF QEASELPVSG QLDDATRARM RQPRCGLEDP FNQKTLKYLL
LGRWRKKHLT FRILNLPSTL PPHTARAALR QAFQDWSNVA PLTFQEVQAG
AADIRLSFHG RQSSYCSNTF DGPGRVLAHA DIPELGSVHF DEDEFWTEGT
YRGVNLRIIA AHEVGHALGL GHSRYSQALM APVYEGYRPH FKLHPDDVAG
IQALYGKKSP VIRDEEEEET ELPTVPPVPT EPSPMPDPCS SELDAMMLGP
RGKTYAFKGD YVWTVSDSGP GPLFRVSALW EGLPGNLDAA VYSPRTQWIH
FFKGDKVWRY INFKMSPGFP KKLNRVEPNL DAALYWPLNQ KVFLFKGSGY
WQWDELARTD FSSYPKPIKG LFTGVPNQPS AAMSWQDGRV YFFKGKVYWR
LNQQLRVEKG YPRNISHNWM HCRPRTIDTT PSGGNTTPSG TGITLDTTLS
ATETTFEY
UCSC uc001sib.5; human. [Q99542-1];

Category

Human MMP19 ELISA Kit