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Human MMP 12 ELISA Kit

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  • Alternative name

    Macrophage metalloelastase ELISA KIT; Macrophage elastase ELISA KIT; ME ELISA KIT; hME ELISA KIT; Matrix metalloproteinase-12 ELISA KIT; MMP-12MMP12 ELISA KIT; HME ELISA KIT; MME ELISA KIT; ME ELISA KIT; hME ELISA KIT; MMP-12 ELISA KIT
  • Catalog
    E023766
  • species
    Human
  • GeneMMP 12
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MMP-12. No significant cross-reactivity or interference between MMP-12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-12 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman MMP 12 ELISA Kit allows for the in vitro quantitative determination of MMP 12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical    Principle of the assay: MMP-12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-12 antibody and an MMP-12-HRP conjugate. The assay sample and buffer are incubated together with MMP-12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-12 concentration since MMP-12 from samples and MMP-12-HRP conjugate compete for the anti-MMP-12 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-12 from the sample, fewer sites are left to bind MMP-12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-12 concentration in each sample is interpolated from this standard curve.



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