Human MMP10 ELISA Kit

Alternative name
Human SL-2 ELISA Kit;Human matrix metalloproteinase-10 ELISA Kit;Human MMP-10 ELISA Kit;Human Transin-2 ELISA Kit;Human STMY2 ELISA Kit;Human matrix metallopeptidase 10 ELISA Kit;Human stromelysin-2 ELISA Kit;Human matrix metalloprotease 10 ELISA Kit;Human matrix metalloproteinase 10 (stromelysin 2) ELISA Kit;Human transin 2 ELISA Kit;
Catalog
E023760
species
Human
GeneMMP10
Standard Curve
Other Species Mouse Mmp10 ELISA Kit Rat Mmp10 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10). No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 34pg/mL.
Intended UseHuman MMP10 ELISA Kit allows for the in vitro quantitative determination of MMP10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 10 (MMP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 10 (MMP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 10 (MMP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 10 (MMP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Stromelysin-2 Protein information
Uniprot ID MMP10_HUMAN
Uniprot AC P09238; B2R9X9; Q53HH9;
UniGene Hs.2258;
GeneID 4319
KEGG hsa:4319;
Human Stromelysin-2 Protein SEQUENCE
SEQUENCE 476 AA; 54151 MW; 516DCDDFEF92A0D6 CRC64;
MMHLAFLVLL CLPVCSAYPL SGAAKEEDSN KDLAQQYLEK YYNLEKDVKQ
FRRKDSNLIV KKIQGMQKFL GLEVTGKLDT DTLEVMRKPR CGVPDVGHFS
SFPGMPKWRK THLTYRIVNY TPDLPRDAVD SAIEKALKVW EEVTPLTFSR
LYEGEADIMI SFAVKEHGDF YSFDGPGHSL AHAYPPGPGL YGDIHFDDDE
KWTEDASGTN LFLVAAHELG HSLGLFHSAN TEALMYPLYN SFTELAQFRL
SQDDVNGIQS LYGPPPASTE EPLVPTKSVP SGSEMPAKCD PALSFDAIST
LRGEYLFFKD RYFWRRSHWN PEPEFHLISA FWPSLPSYLD AAYEVNSRDT
VFIFKGNEFW AIRGNEVQAG YPRGIHTLGF PPTIRKIDAA VSDKEKKKTY
FFAADKYWRF DENSQSMEQG FPRLIADDFP GVEPKVDAVL QAFGFFYFFS
GSSQFEFDPN ARMVTHILKS NSWLHC
UCSC uc001phg.3; human.;

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Human MMP10 ELISA Kit