Human MMP7 ELISA Kit

Alternative name
Human matrin ELISA Kit;Human matrix metalloproteinase-7 ELISA Kit;Human MMP-7 ELISA Kit;Human pump-1 protease ELISA Kit;Human uterine metalloproteinase ELISA Kit;Human MPSL1 ELISA Kit;Human PUMP1 ELISA Kit;Human PUMP-1 ELISA Kit;Human matrix metallopeptidase 7 ELISA Kit;Human matrilysin ELISA Kit;Human matrix metallopeptidase 7 (matrilysin, uterine) ELISA Kit;Human matrix metalloproteinase 7 (matrilysin, uterine) ELISA Kit;Human uterine matrilysin ELISA Kit;
Catalog
E023752
species
Human
GeneMMP7
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MMP7. No significant cross-reactivity or interference between Human MMP7 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.156 ng/ml
Intended UseHuman MMP7 ELISA Kit allows for the in vitro quantitative determination of MMP7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Matrilysin is a protein that in humans is encoded by the MMP7 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MMP-7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MMP-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Matrilysin Protein information
Uniprot ID MMP7_HUMAN
Uniprot AC P09237; Q9BTK9;
UniGene Hs.2256;
GeneID 4316
KEGG hsa:4316;
Human Matrilysin Protein SEQUENCE
SEQUENCE 267 AA; 29677 MW; F6BD1FC0ADA23603 CRC64;
MRLTVLCAVC LLPGSLALPL PQEAGGMSEL QWEQAQDYLK RFYLYDSETK
NANSLEAKLK EMQKFFGLPI TGMLNSRVIE IMQKPRCGVP DVAEYSLFPN
SPKWTSKVVT YRIVSYTRDL PHITVDRLVS KALNMWGKEI PLHFRKVVWG
TADIMIGFAR GAHGDSYPFD GPGNTLAHAF APGTGLGGDA HFDEDERWTD
GSSLGINFLY AATHELGHSL GMGHSSDPNA VMYPTYGNGD PQNFKLSQDD
IKGIQKLYGK RSNSRKK
UCSC uc001phb.4; human.;

Human MMP7 ELISA Kit

Contact Us