Human MMP27 ELISA Kit

Alternative name
Human MMP-27 ELISA Kit;Human matrix metallopeptidase 27 ELISA Kit;Human matrix metalloproteinase-27 ELISA Kit;Human matrix metalloprotease 27 ELISA Kit;
Catalog
E023749
species
Human
GeneMMP27
SpecificityThis assay has high sensitivity and excellent specificity for detection of human MMP27. No significant cross-reactivity or interference between human MMP27 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MMP27 is typically less than 0.39ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman MMP27 ELISA Kit allows for the in vitro quantitative determination of MMP27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP27 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Matrix metalloproteinase-27 Protein information
Uniprot ID MMP27_HUMAN
Uniprot AC Q9H306; Q6UWK6;
UniGene Hs.534479;
GeneID 64066
KEGG hsa:64066;
Human Matrix metalloproteinase-27 Protein SEQUENCE
SEQUENCE 513 AA; 59026 MW; 941D5B8DA55BD32A CRC64;
MKRLLLLFLF FITFSSAFPL VRMTENEENM QLAQAYLNQF YSLEIEGNHL
VQSKNRSLID DKIREMQAFF GLTVTGKLDS NTLEIMKTPR CGVPDVGQYG
YTLPGWRKYN LTYRIINYTP DMARAAVDEA IQEGLEVWSK VTPLKFTKIS
KGIADIMIAF RTRVHGRCPR YFDGPLGVLG HAFPPGPGLG GDTHFDEDEN
WTKDGAGFNL FLVAAHEFGH ALGLSHSNDQ TALMFPNYVS LDPRKYPLSQ
DDINGIQSIY GGLPKEPAKP KEPTIPHACD PDLTFDAITT FRREVMFFKG
RHLWRIYYDI TDVEFELIAS FWPSLPADLQ AAYENPRDKI LVFKDENFWM
IRGYAVLPDY PKSIHTLGFP GRVKKIDAAV CDKTTRKTYF FVGIWCWRFD
EMTQTMDKGF PQRVVKHFPG ISIRVDAAFQ YKGFFFFSRG SKQFEYDIKT
KNITRIMRTN TWFQCKEPKN SSFGFDINKE KAHSGGIKIL YHKSLSLFIF
GIVHLLKNTS IYQ
UCSC uc001phd.2; human.;

Category

Human MMP27 ELISA Kit

Human MMP-27 ELISA Kit;Human matrix metallopeptidase 27 ELISA Kit;Human matrix metalloproteinase-27 ELISA Kit;Human matrix metalloprotease 27 ELISA Kit;