Human MMP2 ELISA Kit

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  • Alternative name

    Human 72 kDa gelatinase ELISA Kit;Human Gelatinase A ELISA Kit;Human matrix metalloproteinase-2 ELISA Kit;Human MMP-2 ELISA Kit;Human TBE-1 ELISA Kit;Human CLG4A ELISA Kit;Human CLG4 ELISA Kit;Human MMP-II ELISA Kit;Human MONA ELISA Kit;Human matrix metallopeptidase 2 ELISA Kit;Human 72 kDa type IV collagenase ELISA Kit;Human collagenase type IV-A ELISA Kit;Human matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) ELISA Kit;Human matrix metalloproteinase-II ELISA Kit;Human neutrophil gelatinase ELISA Kit;

  • Catalog
    E023744
  • species
    Human
  • GeneMMP2
  • Other Species Mouse Mmp2 ELISA KitBovine MMP2 ELISA KitChicken MMP2 ELISA KitRabbit MMP2 ELISA KitRat Mmp2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MMP2. No significant cross-reactivity or interference between Human MMP2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.195 ng/ml
  • Intended UseHuman MMP2 ELISA Kit allows for the in vitro quantitative determination of MMP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Matrix metalloproteinases (MMPs), also called matrixins, constitute a family of zinc and calcium-dependent endopeptidases that function in the breakdown of extracellular matrix and in the processing of a variety of biological molecules. They play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction and tissue remodeling . They also participate in many pathological processes such as arthritis, cancer and cardiovascular disease . While the amounts of newly synthesized MMPs are regulated mainly at the levels of transcription, the proteolytic activities of existing MMPs are controlled through both the activation of proenzymes or zymogens and the inhibition of active enzymes by endogenous inhibitors such as alpha2-macroglobulins and tissue inhibitors of metalloproteinases (TIMPs). MMP-2 (gelatinase A) is primarily expressed in mesenchymal cells (mainly fibroblasts) during development and tissue regeneration. It is highly expressed in stromal cells surrounding the invading front of metastasizing tumors and associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. It is required for the switch to the angiogenic phenotype in a tumor model and its levels are elevated in tumor endothelium and in urine of patients with a variety of cancers. Together with MMP-9 (gelatinase B), it degrades type IV collagen, the major component of basement membranes and gelatin (denatured collagen). It can also degrade other types of collagens (V, VII and X) as well as elastin and fibronectin. It processes many other molecules, modulating their functions in different pathways. Human and mouse MMP-2 are secreted as 72 kDa proenzymes of 631 and 662 amino acids, respectively. The removal of the pro domain can be initiated by membrane-type MMPs or by serine proteases such as thrombin and activated protein C. The resulting mature and active enzyme consists of a catalytic domain, which is interrupted by three contiguous fibronectin type II-like domains, and a C-terminal, hemopexin-like domain. TIMPs inhibit active MMP-2 through tight, but non-covalent binding of their N-terminal domains to the catalytic domain of MMP-2 in a 1:1 stoichiometry. In addition, TIMP-2 can bind to the proenzyme through interaction between the C-terminal domains of both proteins. Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human PEX Protein information
  • Uniprot ID MMP2_HUMAN
  • Uniprot AC P08253; B2R6U1; B4DWH3; E9PE45; Q9UCJ8;
  • UniGene Hs.513617;
  • GeneID 4313
  • KEGG hsa:4313;
  • Human PEX Protein SEQUENCE
  • SEQUENCE 660 AA; 73882 MW; BC7147DC8B49F289 CRC64;

    MEALMARGAL TGPLRALCLL GCLLSHAAAA PSPIIKFPGD VAPKTDKELA

    VQYLNTFYGC PKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP

    RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARAFQV

    WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG

    VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GKEYNSCTDT

    GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNAEGQP CKFPFRFQGT

    SYDSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV

    FPFTFLGNKY ESCTSAGRSD GKMWCATTAN YDDDRKWGFC PDQGYSLFLV

    AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSQDDIKG IQELYGASPD

    IDLGTGPTPT LGPVTPEICK QDIVFDGIAQ IRGEIFFFKD RFIWRTVTPR

    DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER

    GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP

    GFPKLIADAW NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF

    GSIKSDWLGC

  • UCSC uc002ehz.5; human. [P08253-1];



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