Human MEPE ELISA Kit

Alternative name
Human osteoblast/osteocyte factor 45 ELISA Kit;Human OF45 ELISA Kit;Human matrix extracellular phosphoglycoprotein ELISA Kit;Human matrix, extracellular phosphoglycoprotein with ASARM motif (bone) ELISA Kit;
Catalog
E023725
species
Human
GeneMEPE
Standard Curve
Other Species Mouse MEPE ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MEPE. No significant cross-reactivity or interference between Human MEPE and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 ng/ml
Intended UseHuman MEPE ELISA Kit allows for the in vitro quantitative determination of MEPE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MEPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Matrix extracellular phosphoglycoprotein Protein information
Uniprot ID MEPE_HUMAN
Uniprot AC Q9NQ76; A1A4X9; A8MTA3; D2CFR4; F5H5C5;
UniGene Hs.676357;
GeneID 56955
KEGG hsa:56955;
Human Matrix extracellular phosphoglycoprotein Protein SEQUENCE
SEQUENCE 525 AA; 58419 MW; 0977CA6E871CA9E5 CRC64;
MRVFCVGLLL FSVTWAAPTF QPQTEKTKQS CVEEQRQEEK NKDNIGFHHL
GKRINQELSS KENIVQERKK DLSLSEASEN KGSSKSQNYF TNRQRLNKEY
SISNKENTHN GLRMSIYPKS TGNKGFEDGD DAISKLHDQE EYGAALIRNN
MQHIMGPVTA IKLLGEENKE NTPRNVLNII PASMNYAKAH SKDKKKPQRD
SQAQKSPVKS KSTHRIQHNI DYLKHLSKVK KIPSDFEGSG YTDLQERGDN
DISPFSGDGQ PFKDIPGKGE ATGPDLEGKD IQTGFAGPSE AESTHLDTKK
PGYNEIPERE ENGGNTIGTR DETAKEADAV DVSLVEGSND IMGSTNFKEL
PGREGNRVDA GSQNAHQGKV EFHYPPAPSK EKRKEGSSDA AESTNYNEIP
KNGKGSTRKG VDHSNRNQAT LNEKQRFPSK GKSQGLPIPS RGLDNEIKNE
MDSFNGPSHE NIITHGRKYH YVPHRQNNST RNKGMPQGKG SWGRQPHSNR
RFSSRRRDDS SESSDSGSSS ESDGD
UCSC uc003hqy.4; human. [Q9NQ76-1];

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Category

Human MEPE ELISA Kit

Human osteoblast/osteocyte factor 45 ELISA Kit;Human OF45 ELISA Kit;Human matrix extracellular phosphoglycoprotein ELISA Kit;Human matrix, extracellular phosphoglycoprotein with ASARM motif (bone) ELISA Kit;