Human MELK ELISA Kit

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  • Alternative name

    Human hMELK ELISA Kit;Human protein kinase Eg3 ELISA Kit;Human pEg3 kinase ELISA Kit;Human protein kinase PK38 ELISA Kit;Human HPK38 ELISA Kit;Human tyrosine-protein kinase MELK ELISA Kit;Human KIAA0175 ELISA Kit;Human maternal embryonic leucine zipper kinase ELISA Kit;

  • Catalog
    E023706
  • species
    Human
  • GeneMELK
  • Standard CurveHuman MELK ELISA Kit
  • Other Species Mouse Melk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Maternal Embryonic Leucine Zipper Kinase (MELK). No significant cross-reactivity or interference between Maternal Embryonic Leucine Zipper Kinase (MELK) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.124ng/mL.
  • Intended UseHuman MELK ELISA Kit allows for the in vitro quantitative determination of MELK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Maternal Embryonic Leucine Zipper Kinase (MELK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Maternal Embryonic Leucine Zipper Kinase (MELK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Maternal Embryonic Leucine Zipper Kinase (MELK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Maternal Embryonic Leucine Zipper Kinase (MELK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Maternal embryonic leucine zipper kinase Protein information
  • Uniprot ID MELK_HUMAN
  • Uniprot AC B7Z6Y0; B7Z7Q1; D3DRP8; F5H0Y0; F5H2R4; F5H689; Q7L3C3;
  • UniGene Hs.184339;
  • GeneID 9833
  • KEGG hsa:9833;
  • Human Maternal embryonic leucine zipper kinase Protein SEQUENCE
  • SEQUENCE 651 AA; 74642 MW; 57F05CDC6122E570 CRC64;

    MKDYDELLKY YELHETIGTG GFAKVKLACH ILTGEMVAIK IMDKNTLGSD

    LPRIKTEIEA LKNLRHQHIC QLYHVLETAN KIFMVLEYCP GGELFDYIIS

    QDRLSEEETR VVFRQIVSAV AYVHSQGYAH RDLKPENLLF DEYHKLKLID

    FGLCAKPKGN KDYHLQTCCG SLAYAAPELI QGKSYLGSEA DVWSMGILLY

    VLMCGFLPFD DDNVMALYKK IMRGKYDVPK WLSPSSILLL QQMLQVDPKK

    RISMKNLLNH PWIMQDYNYP VEWQSKNPFI HLDDDCVTEL SVHHRNNRQT

    MEDLISLWQY DHLTATYLLL LAKKARGKPV RLRLSSFSCG QASATPFTDI

    KSNNWSLEDV TASDKNYVAG LIDYDWCEDD LSTGAATPRT SQFTKYWTES

    NGVESKSLTP ALCRTPANKL KNKENVYTPK SAVKNEEYFM FPEPKTPVNK

    NQHKREILTT PNRYTTPSKA RNQCLKETPI KIPVNSTGTD KLMTGVISPE

    RRCRSVELDL NQAHMEETPK RKGAKVFGSL ERGLDKVITV LTRSKRKGSA

    RDGPRRLKLH YNVTTTRLVN PDQLLNEIMS ILPKKHVDFV QKGYTLKCQT

    QSDFGKVTMQ FELEVCQLQK PDVVGIRRQR LKGDAWVYKR LVEDILSSCK

    V

  • UCSC uc003zzn.5; human. [Q14680-1];



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