Human MCC ELISA Kit

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  • Alternative name

    Human Protein MCC ELISA Kit;Human MCC1 ELISA Kit;Human mutated in colorectal cancers ELISA Kit;Human colorectal mutant cancer protein ELISA Kit;

  • Catalog
    E023690
  • species
    Human
  • GeneMCC
  • Standard CurveHuman MCC ELISA Kit
  • Other Species Mouse MCC ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MCC ELISA Kit allows for the in vitro quantitative determination of MCC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    For Sample Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This CMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY CMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CMA antibody and an CMA-HRP conjugate. The assay sample and buffer are incubated together with CMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CMA concentration since CMA from samples and CMA-HRP conjugate compete for the anti-CMA antibody binding site. Since the number of sites is limited, as more sites are occupied by CMA from the sample, fewer sites are left to bind CMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CMA concentration in each sample is interpolated from this standard curve.
  • Human Colorectal mutant cancer protein Protein information
  • Uniprot ID CRCM_HUMAN
  • Uniprot AC P23508; D3DT05; Q6ZR04;
  • UniGene Hs.593171;
  • GeneID 4163
  • KEGG hsa:4163;
  • Human Colorectal mutant cancer protein Protein SEQUENCE
  • SEQUENCE 829 AA; 93027 MW; C90611B2E91A7F60 CRC64;

    MNSGVAMKYG NDSSAELSEL HSAALASLKG DIVELNKRLQ QTERERDLLE

    KKLAKAQCEQ SHLMREHEDV QERTTLRYEE RITELHSVIA ELNKKIDRLQ

    GTTIREEDEY SELRSELSQS QHEVNEDSRS MDQDQTSVSI PENQSTMVTA

    DMDNCSDLNS ELQRVLTGLE NVVCGRKKSS CSLSVAEVDK HIEQLTTASE

    HCDLAIKTVE EIEGVLGRDL YPNLAEERSR WEKELAGLRE ENESLTAMLC

    SKEEELNRTK ATMNAIREER DRLRRRVREL QTRLQSVQAT GPSSPGRLTS

    TNRPINPSTG ELSTSSSSND IPIAKIAERV KLSKTRSESS SSDRPVLGSE

    ISSIGVSSSV AEHLAHSLQD CSNIQEIFQT LYSHGSAISE SKIREFEVET

    ERLNSRIEHL KSQNDLLTIT LEECKSNAER MSMLVGKYES NATALRLALQ

    YSEQCIEAYE LLLALAESEQ SLILGQFRAA GVGSSPGDQS GDENITQMLK

    RAHDCRKTAE NAAKALLMKL DGSCGGAFAV AGCSVQPWES LSSNSHTSTT

    SSTASSCDTE FTKEDEQRLK DYIQQLKNDR AAVKLTMLEL ESIHIDPLSY

    DVKPRGDSQR LDLENAVLMQ ELMAMKEEMA ELKAQLYLLE KEKKALELKL

    STREAQEQAY LVHIEHLKSE VEEQKEQRMR SLSSTSSGSK DKPGKECADA

    ASPALSLAEL RTTCSENELA AEFTNAIRRE KKLKARVQEL VSALERLTKS

    SEIRHQQSAE FVNDLKRANS NLVAAYEKAK KKHQNKLKKL ESQMMAMVER

    HETQVRMLKQ RIALLEEENS RPHTNETSL

  • UCSC uc003kqj.5; human. [P23508-1];


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