Human MAPKAPK3 ELISA Kit

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  • Alternative name

    Human MAPK-activated protein kinase 3 ELISA Kit;Human MAPKAP kinase 3 ELISA Kit;Human MAPKAP-K3 ELISA Kit;Human MAPKAPK-3 ELISA Kit;Human MK-3 ELISA Kit;Human chromosome 3p kinase ELISA Kit;Human 3PK ELISA Kit;Human MAPKAP3 ELISA Kit;Human MDPT3 ELISA Kit;Human mitogen-activated protein kinase-activated protein kinase 3 ELISA Kit;Human MAP kinase-activated protein kinase 3 ELISA Kit;

  • Catalog
    E023659
  • species
    Human
  • GeneMAPKAPK3
  • Other Species Mouse Mapkapk3 ELISA KitBovine MAPKAPK3 ELISA KitRat Mapkapk3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MAPKAPK3. No significant cross-reactivity or interference between Human MAPKAPK3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human MAPKAPK3 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman MAPKAPK3 ELISA Kit allows for the in vitro quantitative determination of MAPKAPK3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MAPKAPK3 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAPKAPK3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human MAPKAPK3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAPKAPK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MAPKAPK3. You can calculate the concentration of Human MAPKAPK3 in the samples by comparing the OD of the samples to the standard curve.
  • Human MAP kinase-activated protein kinase 3 Protein information
  • Uniprot ID MAPK3_HUMAN
  • Uniprot AC Q16644; B5BU67;
  • UniGene Hs.234521; Hs.735013;
  • GeneID 7867
  • KEGG hsa:7867;
  • Human MAP kinase-activated protein kinase 3 Protein SEQUENCE
  • SEQUENCE 382 AA; 42987 MW; 405F958B7F54E6F3 CRC64;

    MDGETAEEQG GPVPPPVAPG GPGLGGAPGG RREPKKYAVT DDYQLSKQVL

    GLGVNGKVLE CFHRRTGQKC ALKLLYDSPK ARQEVDHHWQ ASGGPHIVCI

    LDVYENMHHG KRCLLIIMEC MEGGELFSRI QERGDQAFTE REAAEIMRDI

    GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD AVLKLTDFGF AKETTQNALQ

    TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP PFYSNTGQAI

    SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN

    HPWINQSMVV PQTPLHTARV LQEDKDHWDE VKEEMTSALA TMRVDYDQVK

    IKDLKTSNNR LLNKRRKKQA GSSSASQGCN NQ

  • UCSC uc003day.3; human.;



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