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Human SERPINB5 ELISA Kit

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  • Alternative name

    Human maspin ELISA Kit;Human peptidase inhibitor 5 ELISA Kit;Human PI-5 ELISA Kit;Human PI5 ELISA Kit;Human serpin family B member 5 ELISA Kit;Human serpin B5 ELISA Kit;Human protease inhibitor 5 (maspin) ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 ELISA Kit;Human serpin peptidase inhibitor, clade B (ovalbumin), member 5 ELISA Kit;
  • Catalog
    E023578
  • species
    Human
  • GeneSERPINB5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mammary Serine Protease Inhibitor (Maspin). No significant cross-reactivity or interference between Mammary Serine Protease Inhibitor (Maspin) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.267ng/mL.
  • Intended UseHuman SERPINB5 ELISA Kit allows for the in vitro quantitative determination of SERPINB5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mammary Serine Protease Inhibitor (Maspin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mammary Serine Protease Inhibitor (Maspin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mammary Serine Protease Inhibitor (Maspin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mammary Serine Protease Inhibitor (Maspin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serpin B5 Protein information
  • Uniprot ID SPB5_HUMAN
  • Uniprot AC P36952; B2R6Y4; Q6N0B4; Q8WW89;
  • UniGene Hs.55279;
  • GeneID 5268
  • KEGG hsa:5268;
  • Human Serpin B5 Protein SEQUENCE

  • SEQUENCE 375 AA; 42100 MW; 9F24E18505912804 CRC64;

    MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT

    ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL

    STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD

    NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM

    EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL

    NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED

    TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA

    DHPFIYIIRH NKTRNIIFFG KFCSP

  • UCSC uc002liy.3; human. [P36952-1];



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