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Human ME2 ELISA Kit

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  • Alternative name

    Human NAD-ME ELISA Kit;Human malic enzyme 2 ELISA Kit;Human ODS1 ELISA Kit;Human NAD-dependent malic enzyme, mitochondrial ELISA Kit;Human malate dehydrogenase (oxaloacetate-decarboxylating) ELISA Kit;Human malic enzyme 2, NAD(+)-dependent, mitochondrial ELISA Kit;Human pyruvic-malic carboxylase ELISA Kit;
  • Catalog
    E023539
  • species
    Human
  • GeneME2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2). No significant cross-reactivity or interference between Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman ME2 ELISA Kit allows for the in vitro quantitative determination of ME2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human NAD-dependent malic enzyme, mitochondrial Protein information
  • Uniprot ID MAOM_HUMAN
  • Uniprot AC P23368; B2R8J2; Q9BWL6; Q9BYG1; Q9H4B2;
  • UniGene Hs.233119;
  • GeneID 4200
  • KEGG hsa:4200;
  • Human NAD-dependent malic enzyme, mitochondrial Protein SEQUENCE

  • SEQUENCE 584 AA; 65444 MW; 4CEF9AF89B07D93D CRC64;

    MLSRLRVVST TCTLACRHLH IKEKGKPLML NPRTNKGMAF TLQERQMLGL

    QGLLPPKIET QDIQALRFHR NLKKMTSPLE KYIYIMGIQE RNEKLFYRIL

    QDDIESLMPI VYTPTVGLAC SQYGHIFRRP KGLFISISDR GHVRSIVDNW

    PENHVKAVVV TDGERILGLG DLGVYGMGIP VGKLCLYTAC AGIRPDRCLP

    VCIDVGTDNI ALLKDPFYMG LYQKRDRTQQ YDDLIDEFMK AITDRYGRNT

    LIQFEDFGNH NAFRFLRKYR EKYCTFNDDI QGTAAVALAG LLAAQKVISK

    PISEHKILFL GAGEAALGIA NLIVMSMVEN GLSEQEAQKK IWMFDKYGLL

    VKGRKAKIDS YQEPFTHSAP ESIPDTFEDA VNILKPSTII GVAGAGRLFT

    PDVIRAMASI NERPVIFALS NPTAQAECTA EEAYTLTEGR CLFASGSPFG

    PVKLTDGRVF TPGQGNNVYI FPGVALAVIL CNTRHISDSV FLEAAKALTS

    QLTDEELAQG RLYPPLANIQ EVSINIAIKV TEYLYANKMA FRYPEPEDKA

    KYVKERTWRS EYDSLLPDVY EWPESASSPP VITE

  • UCSC uc002ley.4; human. [P23368-1];



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