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Human MDH2 ELISA Kit

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  • Alternative name

    Human EIEE51 ELISA Kit;Human M-MDH ELISA Kit;Human MDH ELISA Kit;Human MGC:3559 ELISA Kit;Human MOR1 ELISA Kit;Human malate dehydrogenase 2 ELISA Kit;Human malate dehydrogenase, mitochondrial ELISA Kit;Human malate dehydrogenase 2, NAD (mitochondrial) ELISA Kit;Human testicular tissue protein Li 120 ELISA Kit;
  • Catalog
    E023521
  • species
    Human
  • GeneMDH2
  • Standard CurveHuman MDH2 ELISA Kit
  • Other Species Mouse Mdh2 ELISA KitBovine MDH2 ELISA KitMonkey MDH2 ELISA KitPorcine MDH2 ELISA KitRat Mdh2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Malate Dehydrogenase 2 (MDH2). No significant cross-reactivity or interference between Malate Dehydrogenase 2 (MDH2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.28ng/mL.
  • Intended UseHuman MDH2 ELISA Kit allows for the in vitro quantitative determination of MDH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malate Dehydrogenase 2 (MDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malate Dehydrogenase 2 (MDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malate Dehydrogenase 2 (MDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malate Dehydrogenase 2 (MDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Malate dehydrogenase, mitochondrial Protein information
  • Uniprot ID MDHM_HUMAN
  • Uniprot AC P40926; A8K414; B2RE78; B4DE44; E9PDB2; O43682;
  • UniGene Hs.520967;
  • GeneID 4191
  • KEGG hsa:4191;
  • Human Malate dehydrogenase, mitochondrial Protein SEQUENCE

  • SEQUENCE 338 AA; 35503 MW; AAB9F5E5B2FBC8CA CRC64;

    MLSALARPAS AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV

    SRLTLYDIAH TPGVAADLSH IETKAAVKGY LGPEQLPDCL KGCDVVVIPA

    GVPRKPGMTR DDLFNTNATI VATLTAACAQ HCPEAMICVI ANPVNSTIPI

    TAEVFKKHGV YNPNKIFGVT TLDIVRANTF VAELKGLDPA RVNVPVIGGH

    AGKTIIPLIS QCTPKVDFPQ DQLTALTGRI QEAGTEVVKA KAGAGSATLS

    MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETECTYFST PLLLGKKGIE

    KNLGIGKVSS FEEKMISDAI PELKASIKKG EDFVKTLK

  • UCSC uc003ueo.5; human. [P40926-1];


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