Human MIP-3a/CCL20 ELISA Kit

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  • Alternative name

    C-C motif chemokine 20 ELISA KIT; Beta-chemokine exodus-1 ELISA KIT; CC chemokine LARC ELISA KIT; Liver and activation-regulated chemokine ELISA KIT; Macrophage inflammatory protein 3 alpha ELISA KIT; MIP-3-alphaCCL20 ELISA KIT; LARC ELISA KIT; MIP3A ELISA KIT; SCYA20 ELISA KIT; MIP-3-alpha ELISA KIT

  • Catalog
    E023379
  • species
    Human
  • GeneMIP-3a/CCL20
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 4.69pg/ml
  • Intended UseHuman MIP-3a/CCL20 ELISA Kit allows for the in vitro quantitative determination of MIP-3a/CCL20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MIP-3alphaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MIP-3 alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-3alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman MIP-3alphacan be calculated.


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