Human MIP-1alpha ELISA Kit

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  • Alternative name

    C-C motif chemokine 3 ELISA KIT; G0/G1 switch regulatory protein 19-1 ELISA KIT; Macrophage inflammatory protein 1-alpha ELISA KIT; MIP-1-alphaCCL3 ELISA KIT; G0S19-1 ELISA KIT; MIP1A ELISA KIT; SCYA3 ELISA KIT; MIP-1-alpha ELISA KIT

  • Catalog
    E023365
  • species
    Human
  • GeneMIP-1alpha
  • Other Species Human MIP-1alpha / CCL3 ELISA KitMouse MIP-1alpha ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 14.063pg/ml
  • Intended UseHuman MIP-1alpha ELISA Kit allows for the in vitro quantitative determination of MIP-1alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MIP-1alphaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MIP-1alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MIP-1alphacan be calculated.



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