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Human MDC ELISA Kit

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  • Alternative name

    C-C motif chemokine 22 ELISA KIT; CC chemokine STCP-1 ELISA KIT; MDC(1-69) ELISA KIT; Macrophage-derived chemokine ELISA KIT; Small-inducible cytokine A22 ELISA KIT; Stimulated T-cell chemotactic protein 1CCL22 ELISA KIT; MDC ELISA KIT; SCYA22 ELISA KIT
  • Catalog
    E023359
  • species
    Human
  • GeneMDC
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Derived Chemokine (MDC). No significant cross-reactivity or interference between Macrophage Derived Chemokine (MDC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 30pg/mL.
  • Intended UseHuman MDC ELISA Kit allows for the in vitro quantitative determination of MDC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Derived Chemokine (MDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Derived Chemokine (MDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Derived Chemokine (MDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Derived Chemokine (MDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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