Human MDC/CCL22 ELISA Kit

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  • Alternative name

    Macrophage-derived chemokine CCL22 ELISA KIT; Macrophage-derived chemokine CCL22MDC ELISA KIT;

  • Catalog
    E023356
  • species
    Human
  • GeneMDC/CCL22
  • Standard CurveHuman MDC/CCL22 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MDC/CCL22 ELISA Kit allows for the in vitro quantitative determination of MDC/CCL22 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: MDC/CCL22 ELISA kit applies the competitive enzyme inimunoassay technique utilizing a monoclonal anti-MDC/CCL22 antibody and an MDC/CCL22-HRP conjugate. The assay sample and buffer are incubated together with MDC/CCL22-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spec trophotome trie ally at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MDC/CCL22 concentration since MDC/CCL22 from samples and MDC/CCL22-HRP conjugate compete for the anti-MDC/CCL22 antibody binding site. Since the number of sites is limited, as more sites are occupied by MDC/CCL22 from the sample, fewer sites are left to bind MDC/CCL22-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDC/CCL22 concentration in each sample is interpolated from this standard curve.



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