Human CSF1 ELISA Kit

Alternative name
Human CSF-1 ELISA Kit;Human M-CSF ELISA Kit;Human MCSF ELISA Kit;Human lanimostim ELISA Kit;Human colony stimulating factor 1 ELISA Kit;Human macrophage colony-stimulating factor 1 ELISA Kit;Human colony stimulating factor 1 (macrophage) ELISA Kit;Human macrophage colony stimulating factor 1 ELISA Kit;
Catalog
E023327
species
Human
GeneCSF1
Standard Curve
Other Species Mouse Csf1 ELISA Kit Rat Csf1 ELISA Kit
SpecificityNatural and recombinant human M-CSF
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman CSF1 ELISA Kit allows for the in vitro quantitative determination of CSF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for M-CSF has been precoated onto 96-well plates. Standards(E.coli,E33-S190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human M-CSF amount of sample captured in plate. Background: M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene ismapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with224 and 522 amino acids, resulting from alternative splicing3.
Human Processed macrophage colony-stimulating factor 1 Protein information
Uniprot ID CSF1_HUMAN
Uniprot AC P09603; A8K6J5; Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8;
UniGene Hs.173894;
GeneID 1435
KEGG hsa:1435;
Human Processed macrophage colony-stimulating factor 1 Protein SEQUENCE
SEQUENCE 554 AA; 60179 MW; 656B0894F9255AED CRC64;
MTAPGAAGRC PPTTWLGSLL LLVCLLASRS ITEEVSEYCS HMIGSGHLQS
LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR
DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK
NVFNETKNLL DKDWNIFSKN CNNSFAECSS QDVVTKPDCN CLYPKAIPSS
DPASVSPHQP LAPSMAPVAG LTWEDSEGTE GSSLLPGEQP LHTVDPGSAK
QRPPRSTCQS FEPPETPVVK DSTIGGSPQP RPSVGAFNPG MEDILDSAMG
TNWVPEEASG EASEIPVPQG TELSPSRPGG GSMQTEPARP SNFLSASSPL
PASAKGQQPA DVTGTALPRV GPVRPTGQDW NHTPQKTDHP SALLRDPPEP
GSPRISSLRP QGLSNPSTLS AQPQLSRSHS SGSVLPLGEL EGRRSTRDRR
SPAEPEGGPA SEGAARPLPR FNSVPLTDTG HERQSEGSFS PQLQESVFHL
LVPSVILVLL AVGGLLFYRW RRRSHQEPQR ADSPLEQPEG SPLTQDDRQV
ELPV
UCSC uc001dyt.3; human. [P09603-1];

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Human CSF1 ELISA Kit