Human LOX ELISA Kit

Alternative name
Human lysyl oxidase ELISA Kit;Human AAT10 ELISA Kit;Human protein-lysine 6-oxidase ELISA Kit;
Catalog
E023293
species
Human
GeneLOX
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human LOX. No significant cross-reactivity or interference between Human LOX and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.156 ng/ml
Intended UseHuman LOX ELISA Kit allows for the in vitro quantitative determination of LOX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HnRNP I/PTB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HnRNP I/PTB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HnRNP I/PTB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HnRNP I/PTB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein-lysine 6-oxidase Protein information
Uniprot ID LYOX_HUMAN
Uniprot AC P28300; B2R5Q3; Q5FWF0;
UniGene Hs.102267;
GeneID 4015
KEGG hsa:4015;
Human Protein-lysine 6-oxidase Protein SEQUENCE
SEQUENCE 417 AA; 46944 MW; 6412A78443E03F04 CRC64;
MRFAWTVLLL GPLQLCALVH CAPPAAGQQQ PPREPPAAPG AWRQQIQWEN
NGQVFSLLSL GSQYQPQRRR DPGAAVPGAA NASAQQPRTP ILLIRDNRTA
AARTRTAGSS GVTAGRPRPT ARHWFQAGYS TSRAREAGAS RAENQTAPGE
VPALSNLRPP SRVDGMVGDD PYNPYKYSDD NPYYNYYDTY ERPRPGGRYR
PGYGTGYFQY GLPDLVADPY YIQASTYVQK MSMYNLRCAA EENCLASTAY
RADVRDYDHR VLLRFPQRVK NQGTSDFLPS RPRYSWEWHS CHQHYHSMDE
FSHYDLLDAN TQRRVAEGHK ASFCLEDTSC DYGYHRRFAC TAHTQGLSPG
CYDTYGADID CQWIDITDVK PGNYILKVSV NPSYLVPESD YTNNVVRCDI
RYTGHHAYAS GCTISPY
UCSC uc003ksu.4; human.;

Category

Human LOX ELISA Kit

Human lysyl oxidase ELISA Kit;Human AAT10 ELISA Kit;Human protein-lysine 6-oxidase ELISA Kit;