SensitivityThe Zero-standard was measured 21 times. The detection limit was set as B0 + 1.645*SD and estimated to be 0.144 ng/ml.
Intended UseHuman LYZ ELISA Kit allows for the in vitro quantitative determination of LYZ , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology; Gastroenterology; Research Assays; Assay Categories
Product Description specificalPrinciple of the assay: The assay utilizes the sandwich technique with two selected antibodies that recognize human lysozyme. Calibrator, controls and diluted samples, which are assayed for human lysozyme, are added into the wells of a micro plate coated with a high affine anti-human lysozyme antibody. During the first incubation step, lysozyme is bound by the immobilized antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added into each microtiter well and a "sandwich" of capture antibody - human lysozyme - peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the enzymatic reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of lysozyme. Samples are determined by referring their optical density to a lot-dependent master calibration curve and the use of the calibrator that is run with each test.
Background: Lysozyme (muramidase) is a protein with a molecular weight of approx. 15 kDa and belongs to the group of alkaline glycosidases. Lysozyme is produced by granulocytes, monocytes and macrophages. The main source for faecal Lysozyme are the intestinal granulocytes. Lysozyme can be detected in all cells of the inflammatory infiltrate during an acute attack of Crohn's disease. To some extent, Lysozyme is also secreted actively by mononuclear cells into the bowel lumen.