Human LPAR4 ELISA Kit

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  • Alternative name

    Human LPA receptor 4 ELISA Kit;Human LPA-4 ELISA Kit;Human G-protein coupled receptor 23 ELISA Kit;Human P2Y purinoceptor 9 ELISA Kit;Human P2Y9 ELISA Kit;Human P2Y5-like receptor ELISA Kit;Human purinergic receptor 9 ELISA Kit;Human GPR23 ELISA Kit;Human LPA4 ELISA Kit;Human P2RY9 ELISA Kit;Human P2Y5-LIKE ELISA Kit;Human lysophosphatidic acid receptor 4 ELISA Kit;

  • Catalog
    E023228
  • species
    Human
  • GeneLPAR4
  • Other Species Mouse Lpar4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman LPAR4 ELISA Kit allows for the in vitro quantitative determination of LPAR4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LPAR4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LPAR4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LPAR4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LPAR4 can be calculated.
  • Human Lysophosphatidic acid receptor 4 Protein information
  • Uniprot ID LPAR4_HUMAN
  • Uniprot AC Q99677; B2RAC7; O15132; Q502U9; Q6NSP5;
  • UniGene Hs.522701;
  • GeneID 2846
  • KEGG hsa:2846;
  • Human Lysophosphatidic acid receptor 4 Protein SEQUENCE
  • SEQUENCE 370 AA; 41895 MW; 20857F52A3929E48 CRC64;

    MGDRRFIDFQ FQDSNSSLRP RLGNATANNT CIVDDSFKYN LNGAVYSVVF

    ILGLITNSVS LFVFCFRMKM RSETAIFITN LAVSDLLFVC TLPFKIFYNF

    NRHWPFGDTL CKISGTAFLT NIYGSMLFLT CISVDRFLAI VYPFRSRTIR

    TRRNSAIVCA GVWILVLSGG ISASLFSTTN VNNATTTCFE GFSKRVWKTY

    LSKITIFIEV VGFIIPLILN VSCSSVVLRT LRKPATLSQI GTNKKKVLKM

    ITVHMAVFVV CFVPYNSVLF LYALVRSQAI TNCFLERFAK IMYPITLCLA

    TLNCCFDPFI YYFTLESFQK SFYINAHIRM ESLFKTETPL TTKPSLPAIQ

    EEVSDQTTNN GGELMLESTF

  • UCSC uc010nme.4; human.;


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