Human LFA1a ELISA Kit

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  • Catalog
    E023149
  • species
    Human
  • GeneLFA1a
  • Standard CurveHuman LFA1a ELISA Kit
  • Other Species Mouse LFA1a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Lymphocyte Function Associated Antigen 1 Alpha (LFA1a). No significant cross-reactivity or interference between Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman LFA1a ELISA Kit allows for the in vitro quantitative determination of LFA1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 1 Alpha (LFA1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 1 Alpha (LFA1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 1 Alpha (LFA1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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