Human LYPD2 ELISA Kit

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  • Alternative name

    Human LYPDC2 ELISA Kit;Human UNQ430 ELISA Kit;Human LY6/PLAUR domain containing 2 ELISA Kit;Human ly6/PLAUR domain-containing protein 2 ELISA Kit;Human RGTR430 ELISA Kit;

  • Catalog
    E023102
  • species
    Human
  • GeneLYPD2
  • Standard CurveHuman LYPD2 ELISA Kit
  • Other Species Mouse Lypd2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LYPD2. No significant cross-reactivity or interference between LYPD2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LYPD2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman LYPD2 ELISA Kit allows for the in vitro quantitative determination of LYPD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: LYPD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LYPD2 antibody and an LYPD2-HRP conjugate. The assay sample and buffer are incubated together with LYPD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LYPD2 concentration since LYPD2 from samples and LYPD2-HRP conjugate compete for the anti-LYPD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LYPD2 from the sample, fewer sites are left to bind LYPD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LYPD2 concentration in each sample is interpolated from this standard curve.
  • Human Ly6/PLAUR domain-containing protein 2 Protein information
  • Uniprot ID LYPD2_HUMAN
  • Uniprot AC Q6UXB3; A8K2R6; Q0VD64; Q0VF31;
  • UniGene Hs.441280;
  • GeneID 137797
  • KEGG hsa:137797;
  • Human Ly6/PLAUR domain-containing protein 2 Protein SEQUENCE
  • SEQUENCE 125 AA; 13115 MW; F727D41D50F57756 CRC64;

    MRGTRLALLA LVLAACGELA PALRCYVCPE PTGVSDCVTI ATCTTNETMC

    KTTLYSREIV YPFQGDSTVT KSCASKCKPS DVDGIGQTLP VSCCNTELCN

    VDGAPALNSL HCGALTLLPL LSLRL

  • UCSC uc003ywz.4; human.;



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